UbcH7/UBE2L3 Antibody (3B7) - Azide and BSA Free

Novus Biologicals | Catalog # H00007332-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B7

Format

Azide and BSA Free
View all UbcH7/UBE2L3 Primary Antibodies »

Product Specifications

Immunogen

UBE2L3 (AAH53368, 1 a.a. ~ 154 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD

Specificity

UBE2L3 - ubiquitin-conjugating enzyme E2L 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for UbcH7/UBE2L3 Antibody (3B7) - Azide and BSA Free

Western Blot: UbcH7/UBE2L3 Antibody (3B7) [H00007332-M01]

Western Blot: UbcH7/UBE2L3 Antibody (3B7) [H00007332-M01]

Western Blot: UbcH7/UBE2L3 Antibody (3B7) [H00007332-M01] - UBE2L3 monoclonal antibody (M01), clone 3B7 Analysis of UBE2L3 expression in K-562.
Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody (3B7) [H00007332-M01]

Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody (3B7) [H00007332-M01]

Immunocytochemistry/Immunofluorescence: UbcH7/UBE2L3 Antibody (3B7) [H00007332-M01] - Analysis of monoclonal antibody to UBE2L3 on HeLa cell. Antibody concentration 10 ug/ml.

Applications for UbcH7/UBE2L3 Antibody (3B7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH7/UBE2L3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

E2-F1, L-UBC, UBCE7, UbcM4, UBE2L3

Entrez Gene IDs

7332 (Human)

Gene Symbol

UBE2L3

OMIM

603721 (Human)

UniProt

AAH53368 (Human)

Additional UbcH7/UBE2L3 Products

Product Documents for UbcH7/UBE2L3 Antibody (3B7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for UbcH7/UBE2L3 Antibody (3B7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail