UbcH7/UBE2L3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-30995

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Predicted:

Bovine (100%), Chicken (100%), Porcine (100%), Rhesus Macaque (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UbcH7/UBE2L3 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human UbcH7/UBE2L3. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (100%) and Xenopus Tropicalis (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UbcH7/UBE2L3 Antibody - BSA Free

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995]

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995]

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody.
Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP1-30995]

Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP1-30995]

Immunocytochemistry/Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP1-30995] - Analysis of HeLa, using NBP1-30995 at 1:200 dilution.
Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NBP1-30995]

Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NBP1-30995]

Immunohistochemistry-Paraffin: UbcH7/UBE2L3 Antibody [NBP1-30995] - A549 xenograft, using UBE2L3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995]

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995]

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - A. 30 ug Neuro2A whole cell lysate/extract, B. 30 ug GL261 whole cell lysate/extract, C. 30 ug C8D30 whole cell lysate/extract 15 % SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995]

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995]

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995]

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995]

Western Blot: UbcH7/UBE2L3 Antibody [NBP1-30995] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody.
Immunoprecipitation: UbcH7/UBE2L3 Antibody [NBP1-30995]

Immunoprecipitation: UbcH7/UBE2L3 Antibody [NBP1-30995]

Immunoprecipitation: UbcH7/UBE2L3 Antibody [NBP1-30995] - Immunoprecipitation of UbcH7/UBE2L3 protein from HeLa whole cell extracts using 5 ug of UbcH7/UBE2L3 antibody (NBP1-30995).Western blot analysis was performed using UbcH7/UBE2L3 antibody (NBP1-30995).EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Applications for UbcH7/UBE2L3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 40% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH7/UBE2L3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

E2-F1, L-UBC, UBCE7, UbcM4, UBE2L3

Gene Symbol

UBE2L3

Additional UbcH7/UBE2L3 Products

Product Documents for UbcH7/UBE2L3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UbcH7/UBE2L3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail