UbcH7/UBE2L3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20786

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UbcH7/UBE2L3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UbcH7/UBE2L3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UbcH7/UBE2L3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP2-20786]

Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody [NBP2-20786]

Immunocytochemistry/Immunofluorescence: Ube2L3 Antibody [NBP2-20786] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786]

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786]

Western Blot: Ube2L3 Antibody [NBP2-20786] - Sample (30 ug of whole cell lysate) A: JurKat B: Hela 12% SDS PAGE gel, diluted at 1:1000.
UbcH7/UBE2L3 Antibody

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] -

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] - Whole zebrafish extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000.
UbcH7/UBE2L3 Antibody

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] -

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000.
UbcH7/UBE2L3 Antibody

Immunohistochemistry: UbcH7/UBE2L3 Antibody [NBP2-20786] -

Immunohistochemistry: UbcH7/UBE2L3 Antibody [NBP2-20786] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using UbcH7/UBE2L3 antibody [N1C3] (NBP2-20786) at 1:300 dilution.
UbcH7/UBE2L3 Antibody - BSA Free

Western Blot: UbcH7/UBE2L3 Antibody [NBP2-20786] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (NBP2-20786) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for UbcH7/UBE2L3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-20786 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH7/UBE2L3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

E2-F1, L-UBC, UBCE7, UbcM4, UBE2L3

Gene Symbol

UBE2L3

UniProt

P68036 (Human)

Additional UbcH7/UBE2L3 Products

Product Documents for UbcH7/UBE2L3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UbcH7/UBE2L3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for UbcH7/UBE2L3 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • Name: Youwei Zhang
    Application: Western Blot
    Sample Tested: WHOLE CELL EXTRACTS
    Species: Human
    Verified Customer | Posted 11/26/2014
    WHOLE CELL EXTRACTS
    UbcH7/UBE2L3 Antibody - BSA Free NBP2-20786

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Protocols

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail