UBE2E3 Antibody (OTI7E8)

Novus Biologicals | Catalog # NBP2-03819

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7E8
View all UBE2E3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2E3 (NP_006348) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2E3 Antibody (OTI7E8)

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2E3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human prostate tissue using anti-UBE2E3 mouse monoclonal antibody.
Flow Cytometry: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Flow Cytometry: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Flow Cytometry: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2E3 antibody, and then analyzed by flow cytometry.
Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3.
Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Western Blot: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human colon tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human Kidney tissue using anti-UBE2E3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819]

Immunohistochemistry-Paraffin: UBE2E3 Antibody (OTI7E8) [NBP2-03819] - Staining of paraffin-embedded Human pancreas tissue using anti-UBE2E3 mouse monoclonal antibody.

Applications for UBE2E3 Antibody (OTI7E8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Reviewed Applications

Read 1 review rated 5 using NBP2-03819 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2E3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Alternate Names

E2-23K, UBCE4, UbcH9, UbcM2, UBE2E3

Entrez Gene IDs

10477 (Human)

Gene Symbol

UBE2E3

Additional UBE2E3 Products

Product Documents for UBE2E3 Antibody (OTI7E8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UBE2E3 Antibody (OTI7E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UBE2E3 Antibody (OTI7E8)

Customer Reviews for UBE2E3 Antibody (OTI7E8) (1)

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Customer Images

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  • UBE2E3 Antibody (7E8)
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: PC3 prostate cancer cell line
    Species: Mouse
    Verified Customer | Posted 10/11/2016
    UBE2E3 Antibody (OTI7E8) NBP2-03819

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail