UBE2M/Ubc12 Antibody (3C4) - Azide and BSA Free

Novus Biologicals | Catalog # H00009040-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C4

Format

Azide and BSA Free
View all UBE2M/Ubc12 Primary Antibodies »

Product Specifications

Immunogen

UBE2M (AAH58924, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Specificity

UBE2M - ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for UBE2M/Ubc12 Antibody (3C4) - Azide and BSA Free

Western Blot: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01]

Western Blot: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01]

Western Blot: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - UBE2M monoclonal antibody (M01), clone 3C4 Analysis of UBE2M expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01]

Immunocytochemistry/ Immunofluorescence: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01]

Immunocytochemistry/Immunofluorescence: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - Analysis of monoclonal antibody to UBE2M on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - Detection limit for recombinant GST tagged UBE2M is approximately 0.1ng/ml as a capture antibody.

Applications for UBE2M/Ubc12 Antibody (3C4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2M/Ubc12

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Alternate Names

UBC-RS2, UBC12, UbcH12

Entrez Gene IDs

9040 (Human)

Gene Symbol

UBE2M

OMIM

603173 (Human)

UniProt

AAH58924 (Human)

Additional UBE2M/Ubc12 Products

Product Documents for UBE2M/Ubc12 Antibody (3C4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UBE2M/Ubc12 Antibody (3C4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UBE2M/Ubc12 Antibody (3C4) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail