UBE2N/Ubc13 Antibody (10O9Z5)

Novus Biologicals | Catalog # NBP3-16793

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10O9Z5 expressed in HEK293
View all UBE2N/Ubc13 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2N/Ubc13 (P61088). MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE2N/Ubc13 Antibody (10O9Z5)

Western Blot: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793]

Western Blot: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793]

Western Blot: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793] - Western blot analysis of extracts of various cell lines, using UBE2N/Ubc13 Rabbit mAb (NBP3-16793) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793]

Immunocytochemistry/ Immunofluorescence: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793]

Immunocytochemistry/Immunofluorescence: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793] - Immunofluorescence analysis of U-2 OS cells using UBE2N/Ubc13 Rabbit mAb (NBP3-16793) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793]

Immunohistochemistry-Paraffin: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793]

Immunohistochemistry-Paraffin: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793] - Immunohistochemistry of paraffin-embedded human lung cancer using UBE2N/Ubc13 Rabbit mAb (NBP3-16793) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793]

Immunocytochemistry/ Immunofluorescence: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793]

Immunocytochemistry/Immunofluorescence: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793] - Immunofluorescence analysis of NIH-3T3 cells using UBE2N/Ubc13 Rabbit mAb (NBP3-16793) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
UBE2N/Ubc13 Antibody (10O9Z5)

Immunocytochemistry/ Immunofluorescence: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793] -

Immunocytochemistry/ Immunofluorescence: UBE2N/Ubc13 Antibody (10O9Z5) [NBP3-16793] - Immunofluorescence analysis of U-2 OS cells using UBE2N/Ubc13 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for UBE2N/Ubc13 Antibody (10O9Z5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2N/Ubc13

UBC13 is a member of the E2 ubiquitin-conjugating enzyme family also known as Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N and Bendless-like ubiquitin-conjugating enzyme. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBC13 forms a heterodimer with UBE2V2 which then catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. UBC13 also mediates transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation, as well as error-free DNA repair. UBC13 also contributes to the survival of cells after DNA damage.

Alternate Names

BLU, UBC13, UbcH13, UBE2N

Gene Symbol

UBE2N

Additional UBE2N/Ubc13 Products

Product Documents for UBE2N/Ubc13 Antibody (10O9Z5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UBE2N/Ubc13 Antibody (10O9Z5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

NFkB Pathway

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail