UBE4A Antibody (1G8) - Azide and BSA Free

Novus Biologicals | Catalog # H00009354-M08

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G8

Format

Azide and BSA Free
View all UBE4A Primary Antibodies »

Product Specifications

Immunogen

UBE4A (NP_004779, 974 a.a. ~ 1073 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LAERIKSLADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE

Specificity

UBE4A (1G8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for UBE4A Antibody (1G8) - Azide and BSA Free

Western Blot: UBE4A Antibody (1G8) [H00009354-M08]

Western Blot: UBE4A Antibody (1G8) [H00009354-M08]

Western Blot: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of UBE4A expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: UBE4A Antibody (1G8) [H00009354-M08]

Immunocytochemistry/ Immunofluorescence: UBE4A Antibody (1G8) [H00009354-M08]

Immunocytochemistry/Immunofluorescence: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of monoclonal antibody to UBE4A on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: UBE4A Antibody (1G8) [H00009354-M08]

Western Blot: UBE4A Antibody (1G8) [H00009354-M08]

Western Blot: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of UBE4A expression in PC-12.
Western Blot: UBE4A Antibody (1G8) [H00009354-M08]

Western Blot: UBE4A Antibody (1G8) [H00009354-M08]

Western Blot: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of UBE4A expression in K-562.
ELISA: UBE4A Antibody (1G8) [H00009354-M08]

ELISA: UBE4A Antibody (1G8) [H00009354-M08]

ELISA: UBE4A Antibody (1G8) [H00009354-M08] - Detection limit for recombinant GST tagged UBE4A is approximately 3ng/ml as a capture antibody.

Applications for UBE4A Antibody (1G8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE4A

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.

Alternate Names

E4MGC133315, KIAA0126ubiquitination factor E4A (homologous to yeast UFD2), ubiquitination factor E4A (UFD2 homolog, yeast), UBOX2, UFD2ubiquitin conjugation factor E4 A

Entrez Gene IDs

9354 (Human)

Gene Symbol

UBE4A

OMIM

603753 (Human)

UniProt

NP_004779 (Human)

Additional UBE4A Products

Product Documents for UBE4A Antibody (1G8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for UBE4A Antibody (1G8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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