Ubiquitin Conjugation Initiation Kit

Catalog # Availability Size / Price Qty
Product Details
Citations (11)

Ubiquitin Conjugation Initiation Kit Summary

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).


Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Product Datasheets

Background: Ubiquitin

  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Reagents Provided

Citations for Ubiquitin Conjugation Initiation Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Modulation of M2 macrophage polarization by the crosstalk between Stat6 and Trim24
    Authors: T Yu, S Gan, Q Zhu, D Dai, N Li, H Wang, X Chen, D Hou, Y Wang, Q Pan, J Xu, X Zhang, J Liu, S Pei, C Peng, P Wu, S Romano, C Mao, M Huang, X Zhu, K Shen, J Qin, Y Xiao
    Nat Commun, 2019;10(1):4353.  2019
  2. CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer
    Authors: Y Liu, X Zhang, J Lin, Y Chen, Y Qiao, S Guo, Y Yang, G Zhu, Q Pan, J Wang, F Sun
    Cell Death Dis, 2019;10(9):644.  2019
  3. The E3 ubiquitin ligase TRIM7 suppressed hepatocellular carcinoma progression by directly targeting Src protein
    Authors: L Zhu, C Qin, T Li, X Ma, Y Qiu, Y Lin, D Ma, Z Qin, C Sun, X Shen, Y Zhao, L Han
    Cell Death Differ., 2019;0(0):.  2019
  4. Regulation of a distinct activated RIPK1 intermediate bridging complex I and complex II in TNF?-mediated apoptosis
    Authors: P Amin, M Florez, A Najafov, H Pan, J Geng, D Ofengeim, SA Dziedzic, H Wang, VJ Barrett, Y Ito, MJ LaVoie, J Yuan
    Proc. Natl. Acad. Sci. U.S.A., 2018;115(26):E5944-E5953.  2018
  5. Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
    Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
    Cell Rep, 2017;19(1):188-202.  2017
  6. Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
    Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
    Cell Rep, 2017;19(1):188-202.  2017
  7. Gsk3? and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease
    Biochem J, 2016;0(0):.  2016
  8. Intracellular osteopontin stabilizes TRAF3 to positively regulate innate antiviral response
    Authors: K Zhao, M Zhang, L Zhang, P Wang, G Song, B Liu, H Wu, Z Yin, C Gao
    Sci Rep, 2016;6(0):23771.  2016
  9. Arabidopsis ubiquitin-conjugating enzyme UBC22 is required for female gametophyte development and likely involved in Lys11-linked ubiquitination
    Authors: S Wang, L Cao, H Wang
    J Exp Bot, 2016;0(0):.  2016
  10. MARCH1 regulates insulin sensitivity by controlling cell surface insulin receptor levels
    Nat Commun, 2016;7(0):12639.  2016
  11. RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
    Authors: El Khouri E, Le Pavec G, Toledano M, Delaunay-Moisan A
    J Biol Chem, 2013;288(43):31177-91.  2013


No product specific FAQs exist for this product, however you may

View all Activity Assay FAQs

Reviews for Ubiquitin Conjugation Initiation Kit

There are currently no reviews for this product. Be the first to review Ubiquitin Conjugation Initiation Kit and earn rewards!

Have you used Ubiquitin Conjugation Initiation Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review