Ubiquitin Conjugation Initiation Kit

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K-995
Product Details
Citations (11)
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Ubiquitin Conjugation Initiation Kit Summary

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Human

Product Datasheets

Background: Ubiquitin

References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Reagents Provided

Citations for Ubiquitin Conjugation Initiation Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Modulation of M2 macrophage polarization by the crosstalk between Stat6 and Trim24
    Authors: T Yu, S Gan, Q Zhu, D Dai, N Li, H Wang, X Chen, D Hou, Y Wang, Q Pan, J Xu, X Zhang, J Liu, S Pei, C Peng, P Wu, S Romano, C Mao, M Huang, X Zhu, K Shen, J Qin, Y Xiao
    Nat Commun, 2019;10(1):4353.  2019
  2. CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer
    Authors: Y Liu, X Zhang, J Lin, Y Chen, Y Qiao, S Guo, Y Yang, G Zhu, Q Pan, J Wang, F Sun
    Cell Death Dis, 2019;10(9):644.  2019
  3. The E3 ubiquitin ligase TRIM7 suppressed hepatocellular carcinoma progression by directly targeting Src protein
    Authors: L Zhu, C Qin, T Li, X Ma, Y Qiu, Y Lin, D Ma, Z Qin, C Sun, X Shen, Y Zhao, L Han
    Cell Death Differ., 2019;0(0):.  2019
  4. Regulation of a distinct activated RIPK1 intermediate bridging complex I and complex II in TNF?-mediated apoptosis
    Authors: P Amin, M Florez, A Najafov, H Pan, J Geng, D Ofengeim, SA Dziedzic, H Wang, VJ Barrett, Y Ito, MJ LaVoie, J Yuan
    Proc. Natl. Acad. Sci. U.S.A., 2018;115(26):E5944-E5953.  2018
  5. Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
    Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
    Cell Rep, 2017;19(1):188-202.  2017
  6. Keap1/Cullin3 Modulates p62/SQSTM1 Activity via UBA Domain Ubiquitination
    Authors: Y Lee, TF Chou, SK Pittman, AL Keith, B Razani, CC Weihl
    Cell Rep, 2017;19(1):188-202.  2017
  7. Gsk3? and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease
    Biochem J, 2016;0(0):.  2016
  8. Intracellular osteopontin stabilizes TRAF3 to positively regulate innate antiviral response
    Authors: K Zhao, M Zhang, L Zhang, P Wang, G Song, B Liu, H Wu, Z Yin, C Gao
    Sci Rep, 2016;6(0):23771.  2016
  9. Arabidopsis ubiquitin-conjugating enzyme UBC22 is required for female gametophyte development and likely involved in Lys11-linked ubiquitination
    Authors: S Wang, L Cao, H Wang
    J Exp Bot, 2016;0(0):.  2016
  10. MARCH1 regulates insulin sensitivity by controlling cell surface insulin receptor levels
    Nat Commun, 2016;7(0):12639.  2016
  11. RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
    Authors: El Khouri E, Le Pavec G, Toledano M, Delaunay-Moisan A
    J Biol Chem, 2013;288(43):31177-91.  2013

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