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Ubiquitin Conjugation Kit

Catalog #: K-960
5 Citations Datasheet / COA / SDS

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Citations (5)
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Ubiquitin Conjugation Kit Summary

Specifications

Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Rabbit

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COA
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Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citations for Ubiquitin Conjugation Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Plasmodium falciparum contains functional SCF and CRL4 ubiquitin E3 ligases, and CRL4 is critical for cell division and membrane integrity
    Authors: Rizvi, Z;Reddy, G;Gorde, S;Pundir, P;Das, D;Sijwali, P;
    PLoS Pathogens  2024-02-28
  2. E3 Ubiquitin ligase ZNRF4 negatively regulates NOD2 signalling and induces tolerance to MDP
    Authors: P Bist, WS Cheong, A Ng, N Dikshit, BH Kim, NK Pulloor, HJ Khameneh, M Hedl, AR Shenoy, V Balamurali, NBA Malik, M Hong, A Neutzner, KC Chin, KS Kobayashi, A Bertoletti, A Mortellaro, C Abraham, JD MacMicking, RJ Xavier, B Sukumaran
    Nat Commun, 2017-06-28;8(0):15865.  2017-06-28
  3. Deltex2 represses MyoD expression and inhibits myogenic differentiation by acting as a negative regulator of Jmjd1c
    Authors: D Luo, A de Morree, S Boutet, N Quach, V Natu, A Rustagi, TA Rando
    Proc. Natl. Acad. Sci. U.S.A., 2017-03-28;114(15):E3071-E3080.  2017-03-28
  4. The type II deiodinase is retrotranslocated to the cytoplasm and proteasomes via p97/Atx3 complex.
    Authors: Arrojo E Drigo R, Egri P, Jo S, Gereben B, Bianco A
    Mol Endocrinol, 2013-11-06;27(12):2105-15.  2013-11-06
  5. Sequential posttranslational modifications program FEN1 degradation during cell-cycle progression.
    Authors: Guo Z, Kanjanapangka J, Liu N, Liu S, Liu C, Wu Z, Wang Y, Loh T, Kowolik C, Jamsen J, Zhou M, Truong K, Chen Y, Zheng L, Shen B
    Mol Cell, 2012-06-30;47(3):444-56.  2012-06-30

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