UBR4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-28730
![Immunoprecipitation: UBR4 Antibody [NBP1-28730]](https://resources.rndsystems.com/images/products/UBR4-Antibody-Western-Blot-NBP1-28730-img0001.jpg "Immunoprecipitation: UBR4 Antibody [NBP1-28730]")
![Western Blot: UBR4 Antibody [NBP1-28730]](https://resources.rndsystems.com/images/products/UBR4-Antibody-Western-Blot-NBP1-28730-img0002.jpg "Western Blot: UBR4 Antibody [NBP1-28730]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Cited:
Immunoblotting
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1350 and 1400 of human retinoblastoma-associated factor 600 using the numbering given in entry NP_065816.1 (GeneID 23352).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UBR4 Antibody - BSA Free
Western Blot: UBR4 Antibody [NBP1-28730]
Western Blot: UBR4 Antibody [NBP1-28730] - Whole cell lysate from NIH3T3, TCMK-1, 4T1, and CT26.WT cells.![Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730]](https://resources.rndsystems.com/images/products/UBR4-Antibody-Immunohistochemistry-NBP1-28730-img0004.jpg "Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730]")
Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730]
Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p600 used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for UBR4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 to 1:2000
Immunohistochemistry-Paraffin
1:500 to 1:2000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: UBR4
Alternate Names
600 kDa retinoblastoma protein-associated factor, EC 6.3.2, EC 6.3.2.-, KIAA0462E3 ubiquitin-protein ligase UBR4, KIAA1307zinc finger, UBR1 type 1, N-recognin-4, p600, RBAF600FLJ41863, Retinoblastoma-associated factor of 600 kDa, ubiquitin protein ligase E3 component n-recognin 4, Zinc finger UBR1-type protein 1, ZUBR1retinoblastoma-associated factor 600-like protein
Entrez Gene IDs
23352 (Human)
Gene Symbol
UBR4
UniProt
Additional UBR4 Products
Product Documents for UBR4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UBR4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for UBR4 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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