UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-45339

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 31A3

Format

IHC-Prediluted
View all UCH-L1/PGP9.5 Primary Antibodies »

Product Specifications

Immunogen

UCH-L1/PGP9.5 protein from brain (Uniprot: P09936)

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30377377).

Localization

Cytoplasmic, Endoplasmic Reticulum membrane

Marker

pan-Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining seen in cytoplasm, ER and membrane. Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Specimens which have not been fixed in acetic acid/alcohol require pre-treatment with citrate buffer: for example, unmask of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min.
UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted Western Blot: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] -

Western Blot: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] -

Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted.
UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted Western Blot: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] -

Western Blot: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] -

Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted.
UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] -

Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] -

Flow Cytometric Analysis of T98G cells using UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] -

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] -

Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)
UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted Western Blot: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] -

Western Blot: UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted [NBP2-45339] -

Western blot of UchL1 (PGP9.5) in 1) human, 2) Mouse and 3) rat brain lysate using UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted.

Applications for UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: UCH-L1/PGP9.5

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Entrez Gene IDs

7345 (Human); 29545 (Rat)

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for UCH-L1/PGP9.5 Antibody (31A3) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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