UCH-L1/PGP9.5 Antibody (31A3)

Novus Biologicals | Catalog # NB600-1160

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Zebrafish

Cited:

Human, Mouse, Rat, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 31A3
View all UCH-L1/PGP9.5 Primary Antibodies »

Product Specifications

Immunogen

UCH-L1/PGP9.5 protein from brain (Uniprot: P09936)

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30377377).

Localization

Cytoplasmic, Endoplasmic Reticulum membrane

Marker

pan-Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33130)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for UCH-L1/PGP9.5 Antibody (31A3)

Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3)
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining seen in cytoplasm, ER and membrane. Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Specimens which have not been fixed in acetic acid/alcohol require pre-treatment with citrate buffer: for example, unmask of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min.
Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Flow Cytometric Analysis of T98G cells using UCH-L1/PGP9.5 Antibody (31A3)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of UchL1 in 1) human, 2) mouse and 3) rat brain lysate using UchL1 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of PGP9.5 in rat mesenteric artery using anti-PGP9.5 antibody. Image from verified customer review.
Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Electropherogram images of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 lysates(s) respectively.
UCH-L1/PGP9.5 Antibody (31A3)

Immunohistochemistry-Paraffin: Mouse Monoclonal UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

UCH-L1/PGP9.5 Antibody on bovine adult testis at 1:100. Image from a verified customer review.
UCH-L1/PGP9.5 Antibody (31A3)

Flow Cytometry: Mouse Monoclonal UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160]

Flow on single cell suspension of adult bovine testes, UCH-L1/PGP9.5 was used at 1:50 for 30min on ice. Secondary was Alexa Fluor 647 at 1:500 for 20min on ice. Image from a verified customer review.

Applications for UCH-L1/PGP9.5 Antibody (31A3)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

0.5-1.0ug/ml

Immunohistochemistry-Frozen

0.5-1.0ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/mL

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Cerebellum and IMR-32 lysates, separated by Size, antibody dilution of 10 ug/mL, apparent MW was 31 kDa

Reviewed Applications

Read 3 reviews rated 5 using NB600-1160 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: UCH-L1/PGP9.5

UchL1 (ubiquitin C-terminal hydrolase), also known as PGP9.5 (protein gene product 9.5) and PARK5, is a neuronal biomarker and ubquitin system protein (reviewed in Day and Thompson, 2010). UchL1 is a highly conserved antibody and has shown that it is expressed in neurons and neuroendocrine cells in vertebrates where it comprises about 5-10% of soluble cytoplasmic proteins. A minor proportion of UchL1 in brain is tightly bound to the membrane. UchL1 antibody is also expressed in human oocytes and spermatogonia. It is important to note that the use of an antibody as a biomarker in this case does not imply that an antibody defines absolute tissue specificity. Rather, it means that PGP9.5 is expressed in neurons and neuroendrocine cells at significantly higher levels than in other cell types.
Functionally, UchL1 antibody is a thiol protease enzyme that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UchL1 antibody also binds to free monoubiquitin and inhibits monoubiquitin degradation in lysosomes; it may function to stabilize monoubiquitin within neurons. A mutation in the UchL1 gene has been found to cause a form of Parkinson's disease, and this discovery has spurred considerable research interest in UchL1 and to its alternative name of PARK5.
Antibody to UchL1 is widely used as an immunohistochemical marker of nerves and neuroendocrine cells. The UchL1 clone 31A3 antibody stains neuronal cell bodies and axons in the central and peripheral nervous systems as well as small nerve fibers in peripheral tissues, including epidermal tissues (Day & Thompson, 2010). The antibody also stains neuroendrocrine cells in the kidney, pituitary, thyroid, pancreas, gastrointestinal tract, and tumors of the diffuse neuroendocrine system. This antibody has also identified UchL1 expression in renal tubule spermatogonia, testis, ovary, and both pregnant and non-pregnant corpus luteum. In western blots, the antibody identifies UchL1 as a band of approximately 20-30 kDa. The antibody is specific to UchL1 and does not cross-react with the closely related UchL3 protein (Yi et al, 2007).

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Entrez Gene IDs

7345 (Human); 29545 (Rat)

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (31A3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UCH-L1/PGP9.5 Antibody (31A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for UCH-L1/PGP9.5 Antibody (31A3)

Customer Reviews for UCH-L1/PGP9.5 Antibody (31A3) (3)

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • UCH-L1/PGP9.5 Antibody (31A3)
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Adult testis
    Species: Bovine
    Verified Customer | Posted 01/15/2026
    Flow on single cell suspension of adult bovine testes, UCH-L1 was used at 1:50 for 30min on ice. Secondary was Alexa Fluor 647 at 1:500 for 20min on ice.
    UCH-L1/PGP9.5 Antibody (31A3) NB600-1160
  • UCH-L1/PGP9.5 Antibody (31A3)
    Name: Kelsey Lockhart
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Adult testis
    Species: Bovine
    Verified Customer | Posted 12/23/2025
    Worked well with IHC-Paraffin on bovine adult testis at 1:100.
    UCH-L1/PGP9.5 Antibody (31A3) NB600-1160
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Mesenteric artery-whole
    Species: Rat
    Verified Customer | Posted 03/26/2015
    Mesenteric Artery, PGP 9.5 1:250/
    UCH-L1/PGP9.5 Antibody (31A3) NB600-1160

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