UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-33130
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine, Canine, Zebrafish
Applications
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 31A3
Format
Azide and BSA Free
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Product Specifications
Immunogen
UCH-L1/PGP9.5 protein from brain (Uniprot: P09936)
Reactivity Notes
Zebrafish reactivity reported in scientific literature (PMID: 30377377).
Localization
Cytoplasmic, Endoplasmic Reticulum membrane
Marker
pan-Neuronal Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NB600-1160).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free
![Western Blot: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]](https://resources.rndsystems.com/images/products/UCH-L1-PGP9-5-Antibody-31A3-Azide-and-BSA-Free-Western-Blot-NBP2-33130-img0006.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]")
Western Blot: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]
Western Blot: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - analysis of UchL1 in 1) human, 2) mouse and 3) rat brain lysate using UchL1 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and ECL substrate solution were used for this test.![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]](https://resources.rndsystems.com/images/products/UCH-L1-PGP9-5-Antibody-31A3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33130-img0004.jpg "Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]")
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]
Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]](https://resources.rndsystems.com/images/products/UCH-L1-PGP9-5-Antibody-31A3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33130-img0008.jpg "Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]")
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining seen in cytoplasm, ER and membrane. Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedd![Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]](https://resources.rndsystems.com/images/products/UCH-L1-PGP9-5-Antibody-31A3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33130-img0007.jpg "Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]")
Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]
Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - Flow Cytometric Analysis of T98G cells using UCH-L1/PGP9.5 Antibody (31A3)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).![Western Blot: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]](https://resources.rndsystems.com/images/products/UCH-L1-PGP9-5-Antibody-31A3-Azide-and-BSA-Free-Western-Blot-NBP2-33130-img0005.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]")
Western Blot: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]
Western Blot: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3)![Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]](https://resources.rndsystems.com/images/products/UCH-L1-PGP9-5-Antibody-31A3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33130-img0003.jpg "Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]")
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130]
Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - analysis of PGP9.5 in rat mesenteric artery using anti-PGP9.5 antibody. Image from verified customer review.![Simple Western: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]](https://resources.rndsystems.com/images/products/UCH-L1-PGP9-5-Antibody-31A3-Azide-and-BSA-Free-Simple-Western-NBP2-33130-img0001.jpg "Simple Western: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]")
Simple Western: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]
Simple Western: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]](https://resources.rndsystems.com/images/products/UCH-L1-PGP9-5-Antibody-31A3-Azide-and-BSA-Free-Simple-Western-NBP2-33130-img0002.jpg "Simple Western: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]")
Simple Western: UCH-L1/PGP9.5 Antibody (31A3)Azide and BSA Free [NBP2-33130]
Simple Western: UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free [NBP2-33130] - Electropherogram images of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 lysates(s) respectively.Applications for UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free
Application
Recommended Usage
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation
Optimal dilution for a specific application should be determined.
See Simple Western Antibody Database for Simple Western validation
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: UCH-L1/PGP9.5
UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.
Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.
Long Name
Ubiquitin C-terminal Hydrolase L1
Alternate Names
PARK5, PGP9.5, UCHL1
Gene Symbol
UCHL1
UniProt
Additional UCH-L1/PGP9.5 Products
Product Documents for UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UCH-L1/PGP9.5 Antibody (31A3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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