UCP1 Antibody

Novus Biologicals | Catalog # NBP2-20796

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all UCP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UCP1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%), Rabbit (86%), Sheep (83%), Bovine (83%).

Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UCP1 Antibody

Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796]

Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796]

Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796] - Mouse brown adipocyte. UCP1 stained by UCP1 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796]

Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796]

Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796] - Human colon carcinoma, using UCP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
UCP1 Antibody

Western Blot: UCP1 Antibody [NBP2-20796] -

Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (NBP2-20796) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for UCP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Knockout Validated

Reported in scientific publication (PMID: 32713230).

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UCP1

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq]

Long Name

Uncoupling Protein 1

Alternate Names

SLC25A7, Thermogenin

Gene Symbol

UCP1

UniProt

P25874 (Human)

Additional UCP1 Products

Product Documents for UCP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UCP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for UCP1 Antibody

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  • Q: How specific are the UCP1 antibodies to UCP1 than to UCP2 and 3?

    A: None of our UCP1 antibodies have been tested for cross-reactivity with UCP2 or UCP3, so we cannot guarantee that these antibodies will not bind to the other UCP proteins. However, we can determine the likelihood of this cross-reactivity by running sequence alignments using the immunogen sequences. NB100-2828 is the most likely to be specific for UCP1: its short immunogen sequence is shown on its datasheet, and when I run a UniProt BLAST sequence comparison for human proteins it detects a match with UCP1 (100%), but not UCP2 or UCP3. When I use MultAlin to align the immunogen sequence with the UCP2 and UCP3 protein sequences, it shows only low similarity. For the antibodies with product codes beginning H00007350, again the immunogen sequence is shown on the datasheets for the products. In this case, the immunogen sequence (shared between them) is a little longer. When I run the UniProt Blast, the results are 100% UCP1, 66% UCP3 and 58% UCP2. For NBP2-20796, the exact immunogen sequence is not available, but I have run the UniProt Blast on the amino acid range given, and the results are 100% UCP1, 59% UCP2 and 58% UCP3. Percentages in the 50s-60s suggest that the antibody probably won't cross-react, but may.

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