UCP3 Antibody (6B8C6) - BSA Free

Novus Biologicals | Catalog # NBP2-61821

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 6B8C6

Format

BSA Free
View all UCP3 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human UCP3 (AA: 1-113 and 217-312) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

34.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UCP3 Antibody (6B8C6) - BSA Free

Western Blot: UCP3 Antibody (6B8C6)BSA Free [NBP2-61821]

Western Blot: UCP3 Antibody (6B8C6)BSA Free [NBP2-61821]

Western Blot: UCP3 Antibody (6B8C6) [NBP2-61821] - Analysis using UCP3 mAb against human UCP3 (AA: 1-113 and 217-312) recombinant protein. (Expected MW is 24 kDa)
Immunocytochemistry/ Immunofluorescence: UCP3 Antibody (6B8C6) - BSA Free [NBP2-61821]

Immunocytochemistry/ Immunofluorescence: UCP3 Antibody (6B8C6) - BSA Free [NBP2-61821]

Immunocytochemistry/Immunofluorescence: UCP3 Antibody (6B8C6) [NBP2-61821] - Analysis of HL-7702 cells using UCP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Western Blot: UCP3 Antibody (6B8C6)BSA Free [NBP2-61821]

Western Blot: UCP3 Antibody (6B8C6)BSA Free [NBP2-61821]

Western Blot: UCP3 Antibody (6B8C6) [NBP2-61821] - Analysis using UCP3 mAb against HEK293 (1) and UCP3 (AA:1-113 and 217-312)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: UCP3 Antibody (6B8C6) - BSA Free [NBP2-61821]

ELISA: UCP3 Antibody (6B8C6) - BSA Free [NBP2-61821]

ELISA: UCP3 Antibody (6B8C6) [NBP2-61821] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for UCP3 Antibody (6B8C6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCP3

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.;

Long Name

Uncoupling Protein 3 (Mitochondrial, Proton Carrier)

Alternate Names

SLC25A9

Gene Symbol

UCP3

Additional UCP3 Products

Product Documents for UCP3 Antibody (6B8C6) - BSA Free

Certificate of Analysis

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Product Specific Notices for UCP3 Antibody (6B8C6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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