UFD1L Antibody (2A6F3) - BSA Free

Novus Biologicals | Catalog # NBP2-61829

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2A6F3

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human UFD1L (AA: 208-307) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

34.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UFD1L Antibody (2A6F3) - BSA Free

Western Blot: UFD1L Antibody (2A6F3)BSA Free [NBP2-61829]

Western Blot: UFD1L Antibody (2A6F3)BSA Free [NBP2-61829]

Western Blot: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis using UFD1L mAb against HEK293 (1) and UFD1L (AA: 208-307)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

Immunocytochemistry/ Immunofluorescence: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

Immunocytochemistry/Immunofluorescence: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis of Hela cells using UFD1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

Immunohistochemistry-Paraffin: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

Immunohistochemistry-Paraffin: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis of endometrial cancer tissues using UFD1L mouse mAb with DAB staining.
Flow Cytometry: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

Flow Cytometry: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

Flow Cytometry: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis of Hela cells using UFD1L mouse mAb (green) and negative control (red).
Western Blot: UFD1L Antibody (2A6F3)BSA Free [NBP2-61829]

Western Blot: UFD1L Antibody (2A6F3)BSA Free [NBP2-61829]

Western Blot: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis using UFD1L mAb against human UFD1L (AA: 208-307) recombinant protein. (Expected MW is 36.8 kDa)
Western Blot: UFD1L Antibody (2A6F3)BSA Free [NBP2-61829]

Western Blot: UFD1L Antibody (2A6F3)BSA Free [NBP2-61829]

Western Blot: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis using UFD1L mouse mAb against K562 (1), Hela (2), A431 (3), PC-2 (4), and A549 (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

Immunocytochemistry/ Immunofluorescence: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

Immunocytochemistry/Immunofluorescence: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis of Hela cells using UFD1L mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

Immunohistochemistry-Paraffin: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

Immunohistochemistry-Paraffin: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis of esophageal cancer tissues using UFD1L mouse mAb with DAB staining.
ELISA: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

ELISA: UFD1L Antibody (2A6F3) - BSA Free [NBP2-61829]

ELISA: UFD1L Antibody (2A6F3) [NBP2-61829] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for UFD1L Antibody (2A6F3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UFD1L

The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.;

Alternate Names

ATP1C, ATP1G1ATPase, Na+/K+ transporting, gamma 1 polypeptide, FXYD domain containing ion transport regulator 2, FXYD domain-containing ion transport regulator 2, HOMG2, hypomagnesemia 2, renal, MGC12372, Na(+)/K(+) ATPase subunit gamma, Sodium pump gamma chain, sodium/potassium-transporting ATPase subunit gamma, Sodium-potassium-ATPase, gamma polypeptide

Gene Symbol

UFD1

Additional UFD1L Products

Product Documents for UFD1L Antibody (2A6F3) - BSA Free

Certificate of Analysis

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Product Specific Notices for UFD1L Antibody (2A6F3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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