Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ULK1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid peptide near the center of human ULK1. The immunogen is located within amino acids 350 - 400 of ULK1. Amino Acid Squence: CDLVAEAPSAKPPPDSL

Specificity

Two alternatively spliced transcript variants encoding different isoforms have been identified. ULK1 antibody is predicted to not cross-react with ULK2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit ULK1 Antibody - BSA Free (NBP2-41217) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-ULK1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ULK1 Antibody - BSA Free

Western Blot: ULK1 AntibodyBSA Free [NBP2-41217]

Western Blot: ULK1 AntibodyBSA Free [NBP2-41217]

Western Blot: ULK1 Antibody [NBP2-41217] - Analysis of ULK1 in rat brain tissue lysate with ULK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry-Paraffin: ULK1 Antibody - BSA Free [NBP2-41217]

Immunohistochemistry-Paraffin: ULK1 Antibody - BSA Free [NBP2-41217]

Immunohistochemistry-Paraffin: ULK1 Antibody [NBP2-41217] - Rat brain tissue with ULK1 antibody at 2.5 ug/ml.
ULK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ULK1 Antibody - BSA Free [NBP2-41217] -

Immunocytochemistry/ Immunofluorescence: ULK1 Antibody - BSA Free [NBP2-41217] - Immunofluorescence of ULK1 in rat brain tissue with ULK1 antibody at 20 u/ml.

Applications for ULK1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1 ug/mL
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:32788597)

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ULK1

ULK1 belongs to the serine/threonine protein kinase family. It is involved in axon growth and plays an essential role in neurite branching during sensory axon outgrowth. Knockdown of ULK1 results in impaired endocytosis of nerve growth factor (NGF), excessive axon arborization, and severely stunted axon elongation indicating that ULK1 mediates a non clathrin coated endocytosis in sensory growth cones. Knockdown of ULK1 also inhibits the autophagic response. It appears to act as a convergence point for multiple signals that regulate autophagy, and in turn interacts with a large number of autophagy related (Atg) proteins.

Long Name

Serine/threonine-protein kinase ULK1

Alternate Names

ATG1, hATG1, KIAA0722

Gene Symbol

ULK1

Additional ULK1 Products

Product Documents for ULK1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ULK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ULK1 Antibody - BSA Free

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Protocols

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FAQs

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