UNC13B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33037

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UNC13B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UNC13B. The exact sequence is proprietary.

Localization

Cytoplasm, Membrane, Peripheral membrane protein, Cell membrane, Cell junction, synapse

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

181 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UNC13B Antibody - BSA Free

Western Blot: UNC13B Antibody [NBP1-33037]

Western Blot: UNC13B Antibody [NBP1-33037]

Western Blot: UNC13B Antibody [NBP1-33037] - Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: UNC13B Antibody [NBP1-33037]

Immunocytochemistry/ Immunofluorescence: UNC13B Antibody [NBP1-33037]

Immunocytochemistry/Immunofluorescence: UNC13B Antibody [NBP1-33037] - Analysis of methanol-fixed HeLa, using UNC13B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: UNC13B Antibody [NBP1-33037]

Immunohistochemistry-Paraffin: UNC13B Antibody [NBP1-33037]

Immunohistochemistry-Paraffin: UNC13B Antibody [NBP1-33037] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: UNC13B Antibody [NBP1-33037]

Immunocytochemistry/ Immunofluorescence: UNC13B Antibody [NBP1-33037]

Immunocytochemistry/Immunofluorescence: UNC13B Antibody [NBP1-33037] - UNC13B antibody detects UNC13B protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: UNC13B stained by UNC13B antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for UNC13B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UNC13B

This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis.

Long Name

UNC-13 Homolog B

Alternate Names

hmunc13, MUNC13B, Unc13h2

Gene Symbol

UNC13B

Additional UNC13B Products

Product Documents for UNC13B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UNC13B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for UNC13B Antibody - BSA Free

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Protocols

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FAQs

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