UNC13D/Munc 13-4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-57827

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UNC13D Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPRTETYPDRGQCHLQFQLIHKRRATSASRSQPSYTVHLHLLQQLVSHEVTQHEAGSTSWDGSLSPQAATVLFLHATQKDLSD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UNC13D/Munc 13-4 Antibody - BSA Free

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827]

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827]

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827] - Staining in human spleen and kidney tissues using anti-UNC13D antibody. Corresponding UNC13D RNA-seq data are presented for the same tissues.
Western Blot: UNC13D/Munc 13-4 Antibody [NBP2-57827]

Western Blot: UNC13D/Munc 13-4 Antibody [NBP2-57827]

Western Blot: UNC13D/Munc 13-4 Antibody [NBP2-57827] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: UNC13D/Munc 13-4 Antibody [NBP2-57827]

Immunocytochemistry/ Immunofluorescence: UNC13D/Munc 13-4 Antibody [NBP2-57827]

Immunocytochemistry/Immunofluorescence: UNC13D/Munc 13-4 Antibody [NBP2-57827] - Staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827]

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827]

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827] - Staining of human kidney shows low expression as expected.
Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827]

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827]

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827] - Staining of human spleen shows high expression.

Applications for UNC13D/Munc 13-4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. IHC-P, Retrieval method: HIER pH6

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UNC13D

Munc 13-4 encodes a protein that is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis type 3, a genetically heterogeneous, rare autosomal recessive disorder.

Long Name

UNC-13 Homolog D

Alternate Names

FHL3, HLH3, HPLH3, Munc13-4

Gene Symbol

UNC13D

Additional UNC13D Products

Product Documents for UNC13D/Munc 13-4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UNC13D/Munc 13-4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Lysosomal Enzymes

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for UNC13D/Munc 13-4 Antibody - BSA Free

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  • Q: I'm looking Munc13-4 N terminal antibody with related epitope sequence.

    A:

    Unfortunately we do not epitope map our antibodies, but we do have a whole list of antibodies against your target depending on species reactivity and application you want to use them for. Please see this link to our MUNC 13-4 antibodies. All of the immunogen information that is not proprietary, or known should be presented on the datasheet. Often times a range will be provided where the peptide falls within, or if it was raised against a larger recombinant protein as is the case for the one you inquired about we do not know exactly where the binding occurs.

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