UROD Antibody

Novus Biologicals | Catalog # NBP2-26190

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all UROD Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence KARECVGKTVTLQGN corresponding to internal region according to NP_000365.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for UROD Antibody

Western Blot: UROD Antibody [NBP2-26190]

Western Blot: UROD Antibody [NBP2-26190]

Western Blot: UROD Antibody [NBP2-26190] - Staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: UROD Antibody [NBP2-26190]

Immunohistochemistry-Paraffin: UROD Antibody [NBP2-26190]

Immunohistochemistry-Paraffin: UROD Antibody [NBP2-26190] - (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for UROD Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in lysates of K56 (calculated MW of 40.8 kDa band according to NP_000365.3). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UROD

This gene encodes the fifth enzyme of the heme biosynthetic pathway. This enzyme is responsible for catalyzing the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains. Mutations and deficiency in this enzyme are known to cause familial porphyria cutanea tarda and hepatoerythropoetic porphyria

Alternate Names

EC 4.1.1.37, PCT, UPD, URO-D, uroporphyrinogen decarboxylase, uroporphyrinogen III decarboxylase

Entrez Gene IDs

7389 (Human)

Gene Symbol

UROD

Additional UROD Products

Product Documents for UROD Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for UROD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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