USP22 Antibody - BSA Free

Western Blot: USP22 Antibody - BSA Free [NBP1-49644] -

Catalytic activity of USP22 is not a barrier to reprogramming.A Diagram showing USP22 domains and mutated amino acid positions. B Western blot image showing USP22 protein levels after USP22 overexpressions in both non-targeting and USP22 targeting gRNA expressing fibroblasts. Actin was used as a loading control. C Western blot image showing H2Bub protein levels after USP22 overexpressions in both non-targeting and USP22 targeting gRNA expressing fibroblasts. Histones H3 and H2B were used as loading controls. D Fold change in reprogramming efficiency upon USP22 overexpressions in both wild-type and USP22 knockout background. Triangular images above the bars are sections of representative Tra-1-60-stained wells. Error bars indicate the error of mean. n = 3, independent experiments for KR mutations, n = 4 for CA and KQ mutations in USP22 knockout background, and n = 5 for other comparisons. Two-sided t-test was performed between sgNT1 and USP22 sg1 without any overexpression and p-value is 0.0153. Two-sided t-test was performed between USP22 sg1 expressing cells without overexpression and WT, C185A, K129Q, and K129R and p-value is 0.0129, 0.0054, 0.0264, and 0.0052, respectively. E Western blot image showing H2Bub and H2Aub protein levels after ATXN7L3 or ENY2 knockouts in fibroblasts. H2B was used as a loading control. F Fold change in reprogramming efficiency upon ATXN7L3, ENY2, USP27X, or USP51 knockouts. Triangular images above the bars are sections of representative Tra-1-60-stained wells. Error bars indicate the error of mean. n = 5, independent experiments. Two-sided t-test shows p-value smaller than 0.0001 for ENY2 sg1 and sg2 compared to sgNT1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40102626), licensed under a CC-BY license. Not internally tested by Novus Biologicals.