USP28 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82905

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all USP28 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPND

Reactivity Notes

Mouse, Rat (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP28 Antibody - BSA Free

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905]

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905]

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905] - Staining of human heart muscle, kidney, liver and skeletal muscle using Anti-TNNT2 antibody (A) NBP1-82905 shows similar protein distribution across tissues to independent antibody NBP1-82904 (B).
Western Blot: USP28 Antibody [NBP1-82905]

Western Blot: USP28 Antibody [NBP1-82905]

Western Blot: USP28 Antibody [NBP1-82905] - Analysis using Anti-USP28 antibody NBP1-82905 (A) shows similar pattern to independent antibody NBP1-82904 (B).
Immunocytochemistry/ Immunofluorescence: USP28 Antibody [NBP1-82905]

Immunocytochemistry/ Immunofluorescence: USP28 Antibody [NBP1-82905]

Immunocytochemistry/Immunofluorescence: USP28 Antibody [NBP1-82905] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining shown in green.
Western Blot: USP28 Antibody [NBP1-82905]

Western Blot: USP28 Antibody [NBP1-82905]

Western Blot: USP28 Antibody [NBP1-82905] - Analysis in human cell line A-549.
Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905]

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905]

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905] - Staining of human heart muscle shows strong nuclear positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905]

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905]

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905] - Staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905]

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905]

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905] - Staining of human testis shows weak nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905]

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905]

Immunohistochemistry-Paraffin: USP28 Antibody [NBP1-82905] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.

Applications for USP28 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP28

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).

Long Name

Ubiquitin Specific Protease 28

Alternate Names

Deubiquitinating enzyme 28, EC 3.1.2.15, EC 3.4.19.12, KIAA1515ubiquitin carboxyl-terminal hydrolase 28, ubiquitin specific peptidase 28, ubiquitin specific protease 28, ubiquitin thioesterase 28, Ubiquitin thiolesterase 28, Ubiquitin-specific-processing protease 28

Gene Symbol

USP28

Additional USP28 Products

Product Documents for USP28 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for USP28 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for USP28 Antibody - BSA Free

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Protocols

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