USP46 Antibody (CL0363) - BSA Free

Novus Biologicals | Catalog # NBP2-52923

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL0363

Format

BSA Free
View all USP46 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P62068, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for USP46 Antibody (CL0363) - BSA Free

Western Blot: USP46 Antibody (CL0363) [NBP2-52923]

Western Blot: USP46 Antibody (CL0363) [NBP2-52923]

Western Blot: USP46 Antibody (CL0363) [NBP2-52923] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-USP46 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: USP46 Antibody (CL0363) [NBP2-52923]

Immunocytochemistry/ Immunofluorescence: USP46 Antibody (CL0363) [NBP2-52923]

Immunocytochemistry/Immunofluorescence: USP46 Antibody (CL0363) [NBP2-52923] - Staining of U-2 OS cells using the anti-USP46 monoclonal antibody, showing specific staining in nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Western Blot: USP46 Antibody (CL0363) [NBP2-52923]

Western Blot: USP46 Antibody (CL0363) [NBP2-52923]

Western Blot: USP46 Antibody (CL0363) [NBP2-52923] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG

Applications for USP46 Antibody (CL0363) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USP46

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinatedaction of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteineproteases that function as deubiquitinating enzymes (Quesada et al., 2004 (PubMed 14715245)).(supplied by OMIM)

Long Name

Ubiquitin Specific Protease 46

Alternate Names

Deubiquitinating enzyme 46, EC 3.1.2.15, EC 3.4.19.12, FLJ11850, FLJ12552, FLJ14283, FLJ39393, ubiquitin carboxyl-terminal hydrolase 46, ubiquitin specific peptidase 46, ubiquitin specific protease 46, ubiquitin thioesterase 46, Ubiquitin thiolesterase 46, Ubiquitin-specific-processing protease 46

Gene Symbol

USP46

Additional USP46 Products

Product Documents for USP46 Antibody (CL0363) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for USP46 Antibody (CL0363) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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Protocols

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FAQs

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