USP46 Antibody (CL0363) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43971

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL0363

Format

Azide and BSA Free
View all USP46 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P62068, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for USP46 Antibody (CL0363) - Azide and BSA Free

USP46 Antibody (CL0363) - Azide and BSA Free Western Blot: USP46 Antibody [NBP3-43971]

Western Blot: USP46 Antibody [NBP3-43971]

Lane 1: Marker [kDa]
Lane 2: Human cell line U-251 MG
USP46 Antibody (CL0363) - Azide and BSA Free Western Blot: USP46 Antibody [NBP3-43971]

Western Blot: USP46 Antibody [NBP3-43971]

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-USP46 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
USP46 Antibody (CL0363) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: USP46 Antibody [NBP3-43971]

Immunocytochemistry/ Immunofluorescence: USP46 Antibody [NBP3-43971]

Staining of U-2 OS cells using the anti-USP46 monoclonal antibody).

Applications for USP46 Antibody (CL0363) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1 ug/ml
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP46

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinatedaction of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteineproteases that function as deubiquitinating enzymes (Quesada et al., 2004 (PubMed 14715245)).(supplied by OMIM)

Long Name

Ubiquitin Specific Protease 46

Alternate Names

Deubiquitinating enzyme 46, EC 3.1.2.15, EC 3.4.19.12, FLJ11850, FLJ12552, FLJ14283, FLJ39393, ubiquitin carboxyl-terminal hydrolase 46, ubiquitin specific peptidase 46, ubiquitin specific protease 46, ubiquitin thioesterase 46, Ubiquitin thiolesterase 46, Ubiquitin-specific-processing protease 46

Gene Symbol

USP46

Additional USP46 Products

Product Documents for USP46 Antibody (CL0363) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for USP46 Antibody (CL0363) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

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Protocols

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FAQs

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