USP48 Antibody (3G4) - Azide and BSA Free

Novus Biologicals | Catalog # H00084196-M01

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G4

Format

Azide and BSA Free
View all USP48 Primary Antibodies »

Product Specifications

Immunogen

USP48 (NP_115612, 110 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NLELRQALYLCPSTCSDYMLGDGIQEEKDYEPQTICEHLQYLFALLQNSNRRYIDPSGFVKALGLDTGQQQDAQEFSKLFMSLLEDTLSKQKNPDVRNIVQQQFCGEYAY

Reactivity Notes

Other species not tested.

Specificity

USP48 - ubiquitin specific protease 48

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for USP48 Antibody (3G4) - Azide and BSA Free

Western Blot: USP48 Antibody (3G4) [H00084196-M01]

Western Blot: USP48 Antibody (3G4) [H00084196-M01]

Western Blot: USP48 Antibody (3G4) [H00084196-M01] - USP48 monoclonal antibody (M01), clone 3G4 Analysis of USP48 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: USP48 Antibody (3G4) [H00084196-M01]

Immunocytochemistry/ Immunofluorescence: USP48 Antibody (3G4) [H00084196-M01]

Immunocytochemistry/Immunofluorescence: USP48 Antibody (3G4) [H00084196-M01] - HeLa cells were incubated for 1h at 37C with USP48 Antibody, followed by incubation with Donkey anti-mouse AF 594. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: USP48 Antibody (3G4) [H00084196-M01]

Immunohistochemistry-Paraffin: USP48 Antibody (3G4) [H00084196-M01]

Immunohistochemistry-Paraffin: USP48 Antibody (3G4) [H00084196-M01] - Analysis of monoclonal antibody to USP48 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: USP48 Antibody (3G4) [H00084196-M01] - Detection limit for recombinant GST tagged USP48 is approximately 0.3ng/ml as a capture antibody.

Applications for USP48 Antibody (3G4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. USP48 Antibody (3G4) validated for ICC/IF from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using H00084196-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: USP48

This gene encodes a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Ubiquitin Specific Protease 48

Alternate Names

RAP1GA1, synUSP, USP31

Entrez Gene IDs

84196 (Human)

Gene Symbol

USP48

UniProt

NP_115612

Additional USP48 Products

Product Documents for USP48 Antibody (3G4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for USP48 Antibody (3G4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for USP48 Antibody (3G4) - Azide and BSA Free

Customer Reviews for USP48 Antibody (3G4) - Azide and BSA Free (1)

4 out of 5
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Customer Images

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  • USP48 Antibody (3G4)
    Name: Edward van Beelen
    Application: Immunocytochemistry
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 01/17/2020
    HeLa cells were incubated for 1h at 37C with USP48 mou, followed by incubation with Donkey anti-mouse AF 594.
    USP48 Antibody (3G4) - Azide and BSA Free H00084196-M01
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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FAQs

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