USP9x Antibody

Novus Biologicals | Catalog # NBP1-48321

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all USP9x Primary Antibodies »

Product Specifications

Immunogen

Genomic peptide made to an internal region of the human USP9x protein (within residues 1150-1300). [Swiss-Prot Q93008]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: rat (95%) and zebrafish (80%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

270 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for USP9x Antibody

Western Blot: USP9x Antibody [NBP1-48321]

Western Blot: USP9x Antibody [NBP1-48321]

USP9x-Antibody-Western-Blot-NBP1-48321-img0010.jpg
Immunocytochemistry/ Immunofluorescence: USP9x Antibody [NBP1-48321]

Immunocytochemistry/ Immunofluorescence: USP9x Antibody [NBP1-48321]

Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - Immunocytochemical analysis of USP9X in NTERA-2 cells
Immunohistochemistry: USP9x Antibody [NBP1-48321]

Immunohistochemistry: USP9x Antibody [NBP1-48321]

Immunohistochemistry: USP9x Antibody [NBP1-48321] - IHC staining of Usp9x in brain tissue.
Western Blot: USP9x Antibody [NBP1-48321]

Western Blot: USP9x Antibody [NBP1-48321]

Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in Caco-2 whole cell lysates.
Western Blot: USP9x Antibody [NBP1-48321]

Western Blot: USP9x Antibody [NBP1-48321]

Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in NIH/3T3 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: USP9x Antibody [NBP1-48321]

Immunocytochemistry/ Immunofluorescence: USP9x Antibody [NBP1-48321]

Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - ICC/IC analysis of USP9x in mouse ES cell line 129. Image courtesy of anonymous customer product review.

Applications for USP9x Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:25-1:200

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000
Application Notes
This USP9x antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen ~270 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NBP1-48321 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 0.1% BSA, and 30% Glycerol

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: USP9x

USP9x (ubiquitin-specific protease 9, X chromosome) is one of the largest deubiquitinases (DUBs) belonging to the Ub-specific protease family and implicates in processing of ubiquitin precursors as well as of ubiquitinated proteins, thereby playing a key regulatory role at protein turnover level by preventing degradation of proteins through conjugated ubiquitin removal. USP9X substrates include AF-6, beta-catenin, NUAK1, MARK4, ErbB2, EFA6, Smad4, Mcl1, ASK1, and survivin, and USP9X has been involved in several biological processes that include: protein trafficking and tight junction biogenesis in epithelial cells; regulation of the TGF-beta pathway; chromosome alignment and segregation; component of PEX5-mediated peroxisomal protein import pathway. Moreover, USP9X modulates the proteins levels of: alpha-synuclein, a protein involved in the pathogenesis of Parkinson disease; MCL1, an anti-apoptotic protein generally found overexpressed in cancers; some protein kinases involved in stress responses and cellular energy homeostasis; and some ubiquitin-ligases, such as MARCH7 and Itch.

Long Name

Ubiquitin-specific Protease 9, X-linked

Alternate Names

DFFRX, FAF, FAM

Entrez Gene IDs

8239 (Human); 22284 (Mouse)

Gene Symbol

USP9X

UniProt

Q93008 (Human), P70398 (Mouse)

Additional USP9x Products

Product Documents for USP9x Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for USP9x Antibody

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for USP9x Antibody (1)

5 out of 5
1 Customer Rating
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4 Stars
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1 Stars
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Customer Images

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  • Name: Andrea Cerase
    Application: Immunofluorescence
    Sample Tested: Mouse ES cell line (129)
    Species: Mouse
    Verified Customer | Posted 08/15/2011
    USP9x Antibody NBP1-48321

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Protocols

View specific protocols for USP9x Antibody (NBP1-48321):

USP9x Antibody:
Western Blot Protocol
1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer
apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of
proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations
and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10
minutes each.
7. Dilute the rabbit anti-Usp9x primary antibody (NBP1-48321) in blocking buffer and incubate 1 hour at room
temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10
minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers
instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as
required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided
it does not interfere with antibody-antigen binding.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail