Uteroglobin/SCGB1A1 Antibody (JU34-03)
Novus Biologicals | Catalog # NBP2-75705
Recombinant Monoclonal Antibody
![Western Blot: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]](https://resources.rndsystems.com/images/products/Uteroglobin-SCGB1A1-Antibody-JU34-03-Western-Blot-NBP2-75705-img0006.jpg "Western Blot: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JU34-03 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human Uteroglobin/SCGB1A1 aa 42-91 / 91. (SwissProt: P11684 Human; SwissProt: Q06318 Mouse; SwissProt: P17559 Rat)
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Uteroglobin/SCGB1A1 Antibody (JU34-03)
Western Blot: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]
Western Blot: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705] - Western blot analysis of Uteroglobin/SCGB1A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: human lung tissue lysate Lane 3: A549 cell lysate![Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]](https://resources.rndsystems.com/images/products/Uteroglobin-SCGB1A1-Antibody-JU34-03-Immunocytochemistry-Immunofluorescence-NBP2-75705-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]")
Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]
Immunocytochemistry/Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705] - Staining SCGB1A1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]](https://resources.rndsystems.com/images/products/Uteroglobin-SCGB1A1-Antibody-JU34-03-Immunohistochemistry-Paraffin-NBP2-75705-img0008.jpg "Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]")
Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]
Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705] - Analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SCGB1A1 antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.![Flow Cytometry: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]](https://resources.rndsystems.com/images/products/Uteroglobin-SCGB1A1-Antibody-JU34-03-Flow-Cytometry-NBP2-75705-img0001.jpg "Flow Cytometry: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]")
Flow Cytometry: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]
Flow Cytometry: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705] - Analysis of A549 cells with SCGB1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).![Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]](https://resources.rndsystems.com/images/products/Uteroglobin-SCGB1A1-Antibody-JU34-03-Immunocytochemistry-Immunofluorescence-NBP2-75705-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]")
Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]
Immunocytochemistry/Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705] - Staining SCGB1A1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]](https://resources.rndsystems.com/images/products/Uteroglobin-SCGB1A1-Antibody-JU34-03-Immunocytochemistry-Immunofluorescence-NBP2-75705-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]")
Immunocytochemistry/ Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]
Immunocytochemistry/Immunofluorescence: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705] - Staining SCGB1A1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]](https://resources.rndsystems.com/images/products/Uteroglobin-SCGB1A1-Antibody-JU34-03-Immunohistochemistry-Paraffin-NBP2-75705-img0007.jpg "Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]")
Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705]
Immunohistochemistry-Paraffin: Uteroglobin/SCGB1A1 Antibody (JU34-03) [NBP2-75705] - Analysis of paraffin-embedded human trachea tissue with Rabbit anti-Uteroglobin/SCGB1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Applications for Uteroglobin/SCGB1A1 Antibody (JU34-03)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:1,000
Immunohistochemistry-Paraffin
1:1,000
Western Blot
1:500-1:1000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Uteroglobin/SCGB1A1
Alternate Names
Blastokinin, CC10, CC16, CCPBP, CCSP, Clara, PCB-BP, SCGB1A1, UGB
Gene Symbol
SCGB1A1
Additional Uteroglobin/SCGB1A1 Products
Product Documents for Uteroglobin/SCGB1A1 Antibody (JU34-03)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Uteroglobin/SCGB1A1 Antibody (JU34-03)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Uteroglobin/SCGB1A1 Antibody (JU34-03)
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Q: MAB4218 Uteroglobin antibody Can you tell me if it reacts in mouse tissue?
A: This product has not yet been tested with mouse samples and based on the sequence homology (52%) we do not anticipate this product being able to detect the mouse protein. If you are looking for a mouse antibody for this target, we have two products that might be better options: NBP2-75705 and NBP2-90517.
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