V5 Epitope Tag Antibody [HRP]

Novus Biologicals | Catalog # NB600-395

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Negative)

Label

HRP

Antibody Source

Polyclonal Rabbit IgG
View all V5 Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Rabbits were immunized with a synthetic peptide representing amino acid residues 95 to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for V5 Epitope Tag Antibody [HRP]

Western Blot: V5 Epitope Tag Antibody [HRP] [NB600-395] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Applications for V5 Epitope Tag Antibody [HRP]

Application
Recommended Usage

ELISA

1:10000-1:100000

Immunocytochemistry/ Immunofluorescence

1:200-1:500

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.2% BSA

Preservative

0.05% Pro-Clean 400

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: V5 Epitope Tag

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Alternate Names

GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5

Additional V5 Epitope Tag Products

Product Documents for V5 Epitope Tag Antibody [HRP]

Certificate of Analysis

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Product Specific Notices for V5 Epitope Tag Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for V5 Epitope Tag Antibody [HRP]

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Protocols

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