V5 Epitope Tag Antibody - BSA Free

Novus Biologicals | Catalog # NB600-381

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Epitope Tag

Cited:

Human, Mouse, Insect - Drosophila

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoblotting, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunoblotting, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all V5 Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

Rabbits were immunized with a synthetic peptide representing amino acid residues 95 to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit V5 Epitope Tag Antibody - BSA Free (NB600-381) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. Anti-V5 Epitope Tag Antibody: Cited in 67 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for V5 Epitope Tag Antibody - BSA Free

Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-381]

Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-381]

V5-Epitope-Tag-Antibody-Western-Blot-NB600-381-img0013.jpg
Immunocytochemistry/ Immunofluorescence: V5 Epitope Tag Antibody - BSA Free [NB600-381]

Immunocytochemistry/ Immunofluorescence: V5 Epitope Tag Antibody - BSA Free [NB600-381]

Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - ATG2A-V5 overexpression reduces intracellular neutral lipids in THP-1 cells. Scale bars, 10 um. Regulation of phagocyte triglyceride by a STAT-ATG2 pathway controls mycobacterial infection. Nat Commun (2017)
Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-381]

Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-381]

Western Blot: V5 Epitope Tag Antibody [NB600-381] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/mL (1:25,000).
Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-381]

Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-381]

Western Blot: V5 Epitope Tag Antibody [NB600-381] - NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2). NB600-381 was used at 0.2 ug/mL (1:5000).
Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-381]

Western Blot: V5 Epitope Tag AntibodyBSA Free [NB600-381]

Western Blot: V5 Epitope Tag Antibody [NB600-381] - Analysis using the HRP conjugate of NB600-381. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/mL (1:5,000).
Immunocytochemistry/ Immunofluorescence: V5 Epitope Tag Antibody - BSA Free [NB600-381]

Immunocytochemistry/ Immunofluorescence: V5 Epitope Tag Antibody - BSA Free [NB600-381]

Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - HEK293 cells transiently expressing V5-tagged mutant PFN1 stained with V5 Epitope Tag antibody (red). ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: V5 Epitope Tag Antibody - BSA Free [NB600-381]

Immunocytochemistry/ Immunofluorescence: V5 Epitope Tag Antibody - BSA Free [NB600-381]

Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - Drosophila adult midgut expressing a V5-tagged protein (magenta) using the GAL4/UAS system. Nuclei are shown in green. pAb dilution of 1:500. ICC/IF image submitted by a verified customer review.
Immunoprecipitation: V5 Epitope Tag Antibody - BSA Free [NB600-381]

Immunoprecipitation: V5 Epitope Tag Antibody - BSA Free [NB600-381]

Immunoprecipitation: V5 Epitope Tag Antibody [NB600-381] - HEK293T were transfected with V5-KU86 plasmid, followed by V5 antibody IP then Western blot with v5 antibody (NB600-381). Primary antibody at 1:1000, 4C, overnight. IP/WB image submitted by a verified customer review.
V5 Epitope Tag Antibody - BSA Free

Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] -

PRDX proteins bind to HIF-1 alpha & HIF-2 alpha A. HeLa cells were transfected with an expression vector encoding V5-epitope-tagged PRDX2 (PRDX2-V5) & exposed to 1% O2 for 24 h. Whole cell lysate (WCL) was subject to immunoprecipitation (IP) using anti-HIF-1 alpha antibody or control IgG, followed by immunoblot assays with antibody against V5 epitope or HIF-1 alpha. B. HeLa cells were transfected with PRDX2-V5 vector & exposed to 1% O2 for 24 h. The WCL was subject to IP using anti-V5 antibody or control IgG, followed by immunoblot assays with antibody against V5 or HIF-1 alpha. Light IgG: immunoglobulin light chain from the secondary antibody. C. HeLa cells were transfected with vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-1 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-1 alpha. D. HeLa cells were transfected with empty vector (EV) or vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-2 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-2 alpha. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
V5 Epitope Tag Antibody - BSA Free

Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] -

Hypoxia induces the nuclear translocation of PRDX2 & PRDX4HeLa cells were transfected with vector encoding PRDX2-V5 (P2) or PRDX4-V5 (P4), or empty vector (EV), & exposed to 20% or 1% O2 for 48 h. Nuclear & cytosolic fractions were isolated & subject to immunoblot assays with antibodies against HIF-1 alpha, HIF-2 alpha, V5, alpha -tubulin, & histone H3. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
V5 Epitope Tag Antibody - BSA Free

Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] -

PRDX proteins bind to HIF-1 alpha & HIF-2 alpha A. HeLa cells were transfected with an expression vector encoding V5-epitope-tagged PRDX2 (PRDX2-V5) & exposed to 1% O2 for 24 h. Whole cell lysate (WCL) was subject to immunoprecipitation (IP) using anti-HIF-1 alpha antibody or control IgG, followed by immunoblot assays with antibody against V5 epitope or HIF-1 alpha. B. HeLa cells were transfected with PRDX2-V5 vector & exposed to 1% O2 for 24 h. The WCL was subject to IP using anti-V5 antibody or control IgG, followed by immunoblot assays with antibody against V5 or HIF-1 alpha. Light IgG: immunoglobulin light chain from the secondary antibody. C. HeLa cells were transfected with vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-1 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-1 alpha. D. HeLa cells were transfected with empty vector (EV) or vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-2 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-2 alpha. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
V5 Epitope Tag Antibody - BSA Free

Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] -

Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] - (a) Light & fluorescent micrographs of stable HN33.11 cells expressing (A)-GFP or (B)-GFP, respectively. Results from two stable clones of each transfection pool are shown. Cells are treated with vehicle (Dox−) or 1 μg/mL Dox (Dox+). (b) Western blot of homogenates of stable clones showing endogenous production of full-length p75NTR (FLp75), Dox-induced, but “leaky” production of (A) & (B) & of the V5 tag. Control values for each clone do not differ significantly between GFP(−) & GFP(+) cells (Student's t-test). ***P < 0.001 relative to GFP(−) cells. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21904642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
V5 Epitope Tag Antibody - BSA Free

Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] -

Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] - Mapping the PRDX2 & PRDX4 binding domains of HIF-1 alpha A. & B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector & WCL was incubated with purified GST or GST-HIF-1 alpha fusion protein in the presence of glutathione-Sepharose beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for V5 Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:400

Immunohistochemistry

1:500

Immunoprecipitation

1 - 4 ug/mg lysate

Western Blot

1:1000 - 1:30000
Application Notes
Use in IHC reported in scientific literature (PMID: 23785054). Use in Immunoprecipitation reported in scientific literature (PMID 26085104). Use in ICC/IF reported in scientific literature (PMID 23785054). Use in FLOW reported in scientific literature (PMID 28038472). Use in Immunoblotting reported in scientific literature (PMID:34452909).

Reviewed Applications

Read 3 reviews rated 5 using NB600-381 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: V5 Epitope Tag

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Alternate Names

GKPIPNPLLGLDST Epitope Tag, Parainfluenza virus 5 V/P, Paramyxovirus SV5

Additional V5 Epitope Tag Products

Product Documents for V5 Epitope Tag Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for V5 Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for V5 Epitope Tag Antibody - BSA Free

Customer Reviews for V5 Epitope Tag Antibody - BSA Free (3)

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • V5 Epitope Tag Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: 293 cell extract
    Species: Human
    Verified Customer | Posted 11/21/2020
    Western Blot: HEK293T were transfected with V5-KU86 plasmid, followed by V5 antibody IP then blot with v5 antibody (NB600-381). Primary antibody: 1:1000, 4℃, overnight.
    V5 Epitope Tag Antibody - BSA Free NB600-381
  • V5 Epitope Tag Antibody
    Name: Masahiko Takemura
    Application: Immunohistochemistry
    Sample Tested: Adult intestine
    Species: Drosophila
    Verified Customer | Posted 06/10/2017
    Drosophila adult midgut expressing a V5-tagged protein (magenta) using the GAL4/UAS system. Nuclei are shown in green.
    Dilution 1:500
    V5 Epitope Tag Antibody - BSA Free NB600-381
  • Name: Pamela Keagle
    Application: Immunofluorescence
    Sample Tested: HEK293 cells
    Species: Human
    Verified Customer | Posted 07/06/2016
    HEK293 cells transiently expressing V5-tagged mutant PFN1
    V5 Epitope Tag Antibody - BSA Free NB600-381

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Protocols

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FAQs for V5 Epitope Tag Antibody - BSA Free

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    • Q: I can't find any images or the antibody used in publications for this application, but the description says it works in this particular application. Could the company provide additional data?
      A: This antibody has been validated in IHC on both frozen and paraffin-embedded tissues and is 100% guaranteed for this use. Validation came from customer feedback, so unfortunately, these images are not available. I apologize for the inconvenience. If you are concerned about images, you may want to consider NB600-381 or NB100-62264 as these two products both have over 20 publications.
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