Vanilloid R-like 3/TRPV3 Antibody (N15/39) - BSA Free
Novus Biologicals | Catalog # NBP2-12918
![Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]](https://resources.rndsystems.com/images/products/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Western-Blot-NBP2-12918-img0006.jpg "Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # N15/39
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptide amino acids 458-474 (cytoplasmic C-terminus) of rat TrpV3
Localization
Membrane
Specificity
Detects approx 70kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Vanilloid R-like 3/TRPV3 Antibody (N15/39) - BSA Free
Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]
Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.![Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]](https://resources.rndsystems.com/images/products/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunocytochemistry-Immunofluorescence-NBP2-12918-img0008.jpg "Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]")
Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]
Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 Antibody (D) Composite.![Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]](https://resources.rndsystems.com/images/products/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunohistochemistry-NBP2-12918-img0007.jpg "Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]")
Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]
Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis.![Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]](https://resources.rndsystems.com/images/products/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunocytochemistry-Immunofluorescence-NBP2-12918-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]")
Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918]
Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining in all cells. Some intermediate filamentlike staining in some cells.Applications for Vanilloid R-like 3/TRPV3 Antibody (N15/39) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:1000
Western Blot
1:1000
Application Notes
1 ug/ml of TrpV3 Antibody was sufficient for detection of TrpV3 in 10 ug of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Vanilloid R-like 3/TRPV3
Long Name
Vanilloid Receptor Like 3/Transient Receptor Potential Cation Channel 3
Alternate Names
TRPV3, VanilloidR like 3, VanilloidR-like3, VRL3
Gene Symbol
TRPV3
Additional Vanilloid R-like 3/TRPV3 Products
Product Documents for Vanilloid R-like 3/TRPV3 Antibody (N15/39) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Vanilloid R-like 3/TRPV3 Antibody (N15/39) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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