Vanilloid R1/TRPV1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-71774

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Primate, Primate - Callithrix jacchus (Common Marmoset)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Vanilloid R1/TRPV1 Primary Antibodies »

Product Specifications

Immunogen

A genomic peptide made to a C-terminal region of the human TRPV1 protein (within residues 725-839). [Swiss-Prot# Q8NER1]

Localization

Cell junction: synapse. Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Vanilloid R1/TRPV1 Antibody - BSA Free

Immunohistochemistry: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-71774]

Immunohistochemistry: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-71774]

Vanilloid-R1-TRPV1-Antibody-Immunohistochemistry-NBP1-71774-img0010.jpg
Immunocytochemistry/ Immunofluorescence: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-71774]

Immunocytochemistry/ Immunofluorescence: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-71774]

Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NBP1-71774] - TRPV1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-71774]

Immunohistochemistry: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-71774]

Immunohistochemistry: Vanilloid R1/TRPV1 Antibody [NBP1-71774] - Analysis of TRPV1 in mouse dorsal root ganglion using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-71774]

Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-71774]

Immunohistochemistry-Paraffin: Vanilloid R1/TRPV1 Antibody [NBP1-71774] - Human stomach tissue. Positive staining can be observed in gastric neurons. Image from verified customer review.
Vanilloid R1/TRPV1 Antibody - BSA Free

Immunohistochemistry: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-71774] -

The TRPV1+ and TRPV1+CD4+ immune cells in patients with allergic rhinitisA. Immunohistochemical staining of TRPV1 (single staining) and CD4/TRPV1 (double staining) in patients with allergic rhinitis compared with control subjects B. Counts of TRPV1 + cells and TRPV1+ CD4+ cells in each groups. The statistical P values are presented as * (P < 0.05), and ** (P < 0.01).

Applications for Vanilloid R1/TRPV1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200
Application Notes
This TRPV1 antibody is useful for ICC/IF and IHC-paraffin embedded tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-71774 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vanilloid R1/TRPV1

TRPV1, a ligand-gated cation channel expressed in peripheral nociceptors, induces pain when activated by capsaicin. TRPV1 affects the inhibitory control of neurons by integrating multiple noxious signals, which is critical in chronic pain. Blocking TRPV1 is becoming a more common approach to suppression of pathological pain. When inflammation is present, TRPV1 causes an itch response. Dietary salt content can affect the concentration and activity of TRPV1, which in turn can affect kidney activities resulting in renin regulation. TRPV1 is also critically involved in thermal activation and detection of infrared radiation. Finally, TRPV1 is dysfunctional in hypertrophic hearts.

Long Name

Vanilloid Receptor 1/Transient Receptor Potential Cation Channel 1

Alternate Names

Capsaicin Receptor, TRPV1, VanilloidR1, VR1

Entrez Gene IDs

7442 (Human)

Gene Symbol

TRPV1

UniProt

Q8NER1 (Human)

Additional Vanilloid R1/TRPV1 Products

Product Documents for Vanilloid R1/TRPV1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Vanilloid R1/TRPV1 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Vanilloid R1/TRPV1 Antibody - BSA Free

Customer Reviews for Vanilloid R1/TRPV1 Antibody - BSA Free (1)

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  • Vanilloid R1/TRPV1 Antibody
    Name: Feng Li
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Stomach tissue
    Species: Human
    Verified Customer | Posted 01/23/2019
    Positive staining can be observed in gastric neurons. This antibody is very good for immunostaining.
    Vanilloid R1/TRPV1 Antibody - BSA Free NBP1-71774

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Protocols

View specific protocols for Vanilloid R1/TRPV1 Antibody - BSA Free (NBP1-71774):

Vanilloid R1/TRPV1 Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures."

Vanilloid R1/TRPV1 Antibody:
Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

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