VAP-1/AOC3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-89671
![Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NBP1-89671]](https://resources.rndsystems.com/images/products/VAP-1-AOC3-Antibody-Immunohistochemistry-Paraffin-NBP1-89671-img0013.jpg "Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NBP1-89671]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Equine
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DIDQMIFNRELPQASGLLHHCCFYKHRGRNLVTMTTAPRGLQSGDRATWFGLYYNISGAGFFLHHVGLELLVNHKALDPARWTIQKVFYQGRYYDSLAQLEAQFEAGLVNVVLI
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (82%). Human reactivity reported in scientific literature (PMID: 25562318). Equine reactivity reported from a verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for VAP-1/AOC3 Antibody - BSA Free
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NBP1-89671]
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NBP1-89671] - Equine endometrium. All vascular smooth muscle was positively stained. IHC-P image submitted by a verified customer review.![VAP-1/AOC3 Antibody - BSA Free Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]](https://resources.rndsystems.com/images/products/nbp1-89671_rabbit-polyclonal-vap-1-aoc3-antibody-13520251744216.jpg "Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]")
![VAP-1/AOC3 Antibody - BSA Free Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]](https://resources.rndsystems.com/images/products/nbp1-89671_rabbit-polyclonal-vap-1-aoc3-antibody-15520258544774.jpg "Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]")
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]
Staining of human lung shows strong cytoplasmic positivity in pneumocytes.![VAP-1/AOC3 Antibody - BSA Free Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]](https://resources.rndsystems.com/images/products/nbp1-89671_rabbit-polyclonal-vap-1-aoc3-antibody-15520258273020.jpg "Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]")
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]
Staining of human skeletal muscle shows no positivity in myocytes as expected.![VAP-1/AOC3 Antibody - BSA Free Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]](https://resources.rndsystems.com/images/products/nbp1-89671_rabbit-polyclonal-vap-1-aoc3-antibody-15520258515191.jpg "Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]")
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]
Staining of human skin shows no positivity in squamous epithelial cells as expected.![VAP-1/AOC3 Antibody - BSA Free Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]](https://resources.rndsystems.com/images/products/nbp1-89671_rabbit-polyclonal-vap-1-aoc3-antibody-15520258273091.jpg "Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]")
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]
Staining of human prostate shows moderate membranous positivity in smooth muscle cells.![VAP-1/AOC3 Antibody - BSA Free Western Blot: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]](https://resources.rndsystems.com/images/products/nbp1-89671_rabbit-polyclonal-vap-1-aoc3-antibody-15520258564322.jpg "Western Blot: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]")
Western Blot: VAP-1/AOC3 Antibody - BSA Free [NBP1-89671]
Analysis in human lung tissue.Applications for VAP-1/AOC3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Reviewed Applications
Read 1 review rated 5 using NBP1-89671 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VAP-1/AOC3
Long Name
Vascular Adhesion Protein-1
Alternate Names
AOC3, HPAO, SSAO, VAP1
Gene Symbol
AOC3
Additional VAP-1/AOC3 Products
Product Documents for VAP-1/AOC3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for VAP-1/AOC3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for VAP-1/AOC3 Antibody - BSA Free
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5 out of 5
1 Customer Rating
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Application: Immunohistochemistry-ParaffinSample Tested: EndometriumSpecies: EquineVerified Customer | Posted 02/20/2020All vascular smooth muscle was positively stained
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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