Q: Has the staining of NTERA-2 cells using DDX4 mouse mAb been shown in a publication?

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VASA Antibody (2F9H5)

Novus Biologicals | Catalog # NBP1-51586

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 2F9H5

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Product Specifications

Immunogen

Purified recombinant fragment of human VASA expressed in E. Coli.

Marker

Primordial Germ Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VASA Antibody (2F9H5)

Western Blot: VASA Antibody (2F9H5)BSA Free [NBP1-51586]

Western Blot: VASA Antibody (2F9H5) [NBP1-51586] - Analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.

Immunocytochemistry/ Immunofluorescence: VASA Antibody (2F9H5) - BSA Free [NBP1-51586]

Immunocytochemistry/Immunofluorescence: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Immunohistochemistry-Paraffin: VASA Antibody (2F9H5) - BSA Free [NBP1-51586]

Immunohistochemistry-Paraffin: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.

Flow Cytometry: VASA Antibody (2F9H5) - BSA Free [NBP1-51586]

Flow Cytometry: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).

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Applications for VASA Antibody (2F9H5)

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VASA

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development.

Long Name

DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41

Alternate Names

DDX4

Entrez Gene IDs

54514 (Human)

Gene Symbol

DDX4

UniProt

Q9NQI0

Additional VASA Products

Product Documents for VASA Antibody (2F9H5)

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Certificate of Analysis

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Product Specific Notices for VASA Antibody (2F9H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Primordial and Mature Germ Cells

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