VASP [p Ser157] Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05448

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all VASP Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S157 of human VASP (NP_003361.1). RVSNA

Modification

p Ser157

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VASP [p Ser157] Antibody - Azide and BSA Free

Western Blot: VASP [p Ser157] AntibodyAzide and BSA Free [NBP3-05448]

Western Blot: VASP [p Ser157] AntibodyAzide and BSA Free [NBP3-05448]

Western Blot: VASP [p Ser157] Antibody [NBP3-05448] - Western blot analysis of extracts of A431 cells, using VASP antibody (NBP3-05448) at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody - Azide and BSA Free [NBP3-05448]

Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody - Azide and BSA Free [NBP3-05448]

Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded mouse lung using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody - Azide and BSA Free [NBP3-05448]

Immunohistochemistry-Paraffin: VASP [p Ser157 Antibody - Azide and BSA Free [NBP3-05448]

Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded rat brain using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody - Azide and BSA Free [NBP3-05448]

Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody - Azide and BSA Free [NBP3-05448]

Immunohistochemistry-Paraffin: VASP [p Ser157] Antibody [NBP3-05448] - Immunohistochemistry of paraffin-embedded human colon using VASP antibody (NBP3-05448) at dilution of 1:100 (40x lens).
Immunoprecipitation: VASP [p Ser157] Antibody - Azide and BSA Free [NBP3-05448]

Immunoprecipitation: VASP [p Ser157] Antibody - Azide and BSA Free [NBP3-05448]

Immunoprecipitation: VASP [p Ser157] Antibody [NBP3-05448] - Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug VASP pAb (NBP3-05448). Western blot was performed from the immunoprecipitate using VASP pAb (NBP3-05448) at a dilution of 1:1000. A-431 cells were treated by Forskolin (10 uM) at 37C for 30 minutes after serum-starvation overnight.
VASP [p Ser157] Antibody - Azide and BSA Free

Western Blot: VASP [p Ser157] Antibody - Azide and BSA Free [VASP] -

Western Blot: VASP [p Ser157] Antibody - Azide and BSA Free [VASP] - Western blot analysis of lysates from HEL cells using VASP Rabbit pAb at 1:1000 dilution. HEL cells were treated by Forskolin (20 uM) at 37C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 0.8s.

Applications for VASP [p Ser157] Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunohistochemistry

1:50-1:100

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VASP

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Long Name

Vasodilator-stimulated Phosphoprotein

Alternate Names

vasodilator-stimulated phosphoprotein

Gene Symbol

VASP

Additional VASP Products

Product Documents for VASP [p Ser157] Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for VASP [p Ser157] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Platelet Markers

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Protocols

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