VCAM-1/CD106 Antibody (1.4C3) - IHC-Prediluted

Name: VCAM-1/CD106 Antibody (1.4C3) - IHC-Prediluted
Brand: Novus Biologicals
SKU: NBP2-48171
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-48171

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Product Documents

Clone 1.4C3 was used by HLDA to establish CD designation.

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 1.4C3

Format

IHC-Prediluted

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Product Specifications

Immunogen

Stimulated human umbilical vein endothelial cells (HUVEC)

Localization

Cell surface

Marker

Activated Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for VCAM-1/CD106 Antibody (1.4C3) - IHC-Prediluted

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) - IHC-Prediluted [NBP2-48171]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.4C3) - IHC-Prediluted [NBP2-48171] - Formalin-fixed, paraffin-embedded human tonsil stained with VCAM1 / CD106 antibody at 1:100 using peroxidase-conjugate and DAB chromogen. Note membrane staining.

Applications for VCAM-1/CD106 Antibody (1.4C3) - IHC-Prediluted

Application

Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: VCAM-1/CD106

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (1.4C3) - IHC-Prediluted

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Certificate of Analysis

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Product Specific Notices for VCAM-1/CD106 Antibody (1.4C3) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.