VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit

Novus Biologicals | Catalog # AK-5002-NB

Novus Biologicals
100% Guarantee

Key Product Details

Species

Mouse

Applications

ELISA, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Kit Type

AP Immunodetection Kit

Product Summary for VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit

Features:
  • Alkaline phosphatase enzyme detection system
  • Avidin/biotin technology
  • High sensitivity/low background
  • Preferred for tissues that have high endogenous peroxidase activity
  • Offers additional substrate choices
  • Used as a second label in multiple antigen labeling applications

    • Product Specifications

    Application Notes

    This kit is sufficient to stain approximately 1000-2000 sections or fifty 100 cm^2 blots..

    Scientific Data Images for VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit

    Immunohistochemistry: VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

    Immunohistochemistry: VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

    Immunohistochemistry: Vectastain(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB] - Tumor (double label): p53 (m), VECTASTAIN ABC-AP Kit, Vector Blue substrate (blue); Multi-Cytokeratin (m), VECTASTAIN Elite ABC Kit, AEC substrate (red).
    Immunohistochemistry: VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

    Immunohistochemistry: VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

    Immunohistochemistry: Vectastain(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB] - Prostate (double label): Cytokeratin (m), VECTASTAIN ABC-AP Kit, Vector Blue (blue) substrate. CD34, VECTASTAIN ABC-AP Kit, Vector Red (red) substrate.
    Immunohistochemistry: VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

    Immunohistochemistry: VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

    Immunohistochemistry: Vectastain(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB] - Tumor (double label): Cytokeratin, VECTASTAIN Elite ABC Kit, Vector NovaRED (red) substrate. CD34, VECTASTAIN ABC-AP Kit, BCIP/NBT (blue) substrate.
    Immunohistochemistry: VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

    Immunohistochemistry: VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

    Immunohistochemistry: Vectastain(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN ABC-AP Kit, Vector Blue substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown).
    VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]
    VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

    Kit Contents for VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit

    • Biotinylated horse anti-mouse IgG secondary antibody - 1 mL
    • Blocking Serum (Normal Serum) - 3 mL
    • Reagent A (Avidin DH) - 2 mL
    • Reagent B (Biotinylated Alkaline Phosphatase H) - 2 mL

    Formulation, Preparation, and Storage

    Concentration

    Concentration is not relevant for this product. Please see the protocols for proper use of this product.

    Shipping

    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Storage

    Store at 4C. Do not freeze.

    Background: VECTASTAIN(R) ABC anti-Mouse IgG

    With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory. The sensitivity of the VECTASTAI ABC-AP system is comparable to that of the peroxidase VECTASTAIN Elite ABC system. The VECTASTAIN ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity. The system also offers additional substrate color choices

    Additional VECTASTAIN(R) ABC anti-Mouse IgG Products

    Product Documents for VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit

    Certificate of Analysis

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    Product Specific Notices for VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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    Protocols

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    FAQs

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