VECTASTAIN(R) ABC AP Detection Kit

Novus Biologicals | Catalog # AK-5000-NB

Novus Biologicals
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Key Product Details

Species

Non-species specific

Applications

ELISA, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Kit Type

AP Detection Kit

Product Summary for VECTASTAIN(R) ABC AP Detection Kit

Features:
  • Alkaline phosphatase enzyme detection system
  • Avidin/biotin technology
  • High sensitivity/low background
  • Preferred for tissues that have high endogenous peroxidase activity
  • Offers additional substrate choices
  • Used as a second label in multiple antigen labeling applications

    • Product Specifications

    Application Notes

    Reagent A and B when mixed form the ABC-AP complex.
    Kit is sufficient to stain approximately 1000-2000 sections or fifty 100 cm^2 blots..

    Scientific Data Images for VECTASTAIN(R) ABC AP Detection Kit

    Immunohistochemistry: VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB]

    Immunohistochemistry: VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB]

    Immunohistochemistry: VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB] - VECTASTAIN(R) ABC-AP Staining Kit (Alkaline Phosphatase, Standard)
    VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB]
    VECTASTAIN(R) ABC AP Detection Kit [AK-5000-NB]

    Kit Contents for VECTASTAIN(R) ABC AP Detection Kit

    • Reagent A - Avidin DH (2 mL)
    • Reagent B - Biotinylated AP (2 mL)

    Formulation, Preparation, and Storage

    Concentration

    Concentration is not relevant for this product. Please see the protocols for proper use of this product.

    Shipping

    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Storage

    Store at 4C. Do not freeze.

    Background: VECTASTAIN(R) ABC

    With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory. The sensitivity of the VECTASTAIN ABC-AP system is comparable to that of the peroxidase VECTASTAIN Elite ABC system. The VECTASTAIN ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity.

    Additional VECTASTAIN(R) ABC Products

    Product Documents for VECTASTAIN(R) ABC AP Detection Kit

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Product Specific Notices for VECTASTAIN(R) ABC AP Detection Kit

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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    Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    FAQs

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