VEGFR1/Flt-1 Antibody (CL0345) - BSA Free

Novus Biologicals | Catalog # NBP2-30982

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL0345

Format

BSA Free
View all VEGFR1/Flt-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P17948, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Rat (82%), Mouse (80%).

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for VEGFR1/Flt-1 Antibody (CL0345) - BSA Free

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982] - Staining in human placenta and tonsil tissues. Corresponding FLT1 RNA-seq data are presented for the same tissues.
Western Blot: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Western Blot: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Western Blot: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982] - Staining of human kidney shows strong cytoplasmic positivity in endothelial cells.
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in endothelial cells, as well as positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (CL0345) [NBP2-30982] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Applications for VEGFR1/Flt-1 Antibody (CL0345) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR1/Flt-1

Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Gene Symbol

FLT1

UniProt

P17948

Additional VEGFR1/Flt-1 Products

Product Documents for VEGFR1/Flt-1 Antibody (CL0345) - BSA Free

Certificate of Analysis

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Product Specific Notices for VEGFR1/Flt-1 Antibody (CL0345) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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