VEGFR1/Flt-1 Antibody (SY09-09)

Name: VEGFR1/Flt-1 Antibody (SY09-09)
Brand: Novus Biologicals
SKU: NBP3-11864
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-11864

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SY09-09 expressed in HEK293

View all VEGFR1/Flt-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein (Uniprot#: P17948)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

151 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VEGFR1/Flt-1 Antibody (SY09-09)

Western Blot: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864]

Western Blot: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Western blot analysis of VEGFR1/Flt-1 on mouse lung lysates using NBP3-11864 at 1/1,000 dilution.

Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864]

Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - ICC staining VEGFR1/Flt-1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using NBP3-11864. Counter stained with hematoxylin.

Flow Cytometry: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864]

Flow Cytometry: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Flow cytometric analysis of A431 cells with NBP3-11864 at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864]

Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864]

Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using NBP3-11864. Counter stained with hematoxylin.

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Applications for VEGFR1/Flt-1 Antibody (SY09-09)

Application

Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:1000 - 1:2000

Application Notes

Antibody works well in WB, but does not work well in IP according to YCharOS report for Flt-1.

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1X TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Store at -20C.

Background: VEGFR1/Flt-1

Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Gene Symbol

FLT1

Additional VEGFR1/Flt-1 Products

Product Documents for VEGFR1/Flt-1 Antibody (SY09-09)

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Certificate of Analysis

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Product Specific Notices for VEGFR1/Flt-1 Antibody (SY09-09)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.