Doxorubicin hydrochloride

Tocris Bioscience | Catalog # 2252

Antitumor antibiotic agent. Inhibits DNA topoisomerase II
Tocris Bioscience

Key Product Details

Description

Antitumor antibiotic agent. Inhibits DNA topoisomerase II

Alternative Names

Adriamycin,NSC 123127
View all DNA Topoisomerase Inhibitors »

Product Description

Doxorubicin hydrochloride is a 14-hydroxylated version of Daunorubicin (Cat. No. 1467) that is naturally fluorescent. Doxorubicin is an antitumor antibiotic agent that inhibits DNA topoisomerase II. It is a DNA intercalator that inhibits nucleic acid synthesis and induces apoptosis. Doxorubicin reduces intracellular tau levels. Doxorubicin also promotes formation of free radicals for the disruption of membrane lipids and DNA strands. Doxorubicin fluorescence is quenched upon intercalation into the DNA; while binding to histones or partitioning into the phospholipid phase of PEG-phospholipid micelles or hydrophobic cores of polymeric micelles, increases Doxorubicin fluorescence. Doxorubicin fluorescence enables the monitoring of the localization of the drug within lipid bilayers and liposomal delivery systems and interaction of the drug with DNA and other macromolecules, as well as drug efflux pump activities.

Scientific Data Images for Doxorubicin hydrochloride

Doxorubicin treated cardiomyocytes secrete Troponin T

Doxorubicin induces secretion of Troponin T from pluripotent stem cell-derived cardiomyocytes

Cardiomyocytes were treated with increasing doses of Doxorubicin (Catalog # 2252) for 24 hours. High concentration or extended exposure to Doxorubicin is known to induce heart failure. Doxorubicin-induced cardiac failure was quantified by measuring secreted Troponin T, a known biomarker of heart failure. Troponin T was detected via Luminex® bead-based multiplex assay (Catalog # LXSAH). Increased levels of secreted Troponin T are observed with increasing concentrations of Doxorubicin.

doxorubicin decreases Pro-BNP secretion from cardiomyocytes

Pluripotent stem cell-derived cardiomyocytes secrete Pro-BNP in response to small molecule-induction of cardiac hypertrophy

Cardiomyocytes were treated with the vasoconstrictor, ET-1 (Endothelin-1; Catalog # 1160), or one of two L-type calcium channel inhibitors, Doxorubicin (Catalog # 2252) or Verapamil (Catalog # 0654). Levels of the cardiac hypertrophy marker, Pro-Brain Natriuretic Peptide (Pro-BNP), were detected via Luminex® bead-based multiplex assay (Catalog # LXSAH). A substantial increase in Pro-BNP was detected in the medium of cardiomyocytes treated with ET-1, a known inducer of cardiac hypertrophy. Conversely, Doxorubicin and Verapamil decreased Pro-BNP secretion from cardiomyocytes.

Product Specifications for Doxorubicin hydrochloride

Molecular Weight

579.99

Formula

C27H29NO11.HCl

Storage

Desiccate at RT

Purity

≥98% (HPLC)

Chemical Name

10-[(3-Amino-2,3,6-trideoxy-α-L-lyxohexopyranosyl)oxy]-7,8,9,10-tetrahydro-6,8,11-trihydroxy-8-(hydroxyacetyl)-5,12-naphthacenedione hydrochloride

CAS Number

25316-40-9

PubChem ID

443939

InChI Key

MWWSFMDVAYGXBV-RUELKSSGSA-N

SMILES

OC1=C(C[C@]([C@](CO)=O)(O)C[C@@H]4O[C@@]5([H])C[C@H](N)[C@H](O)[C@H](C)O5)C4=C(O)C3=C1C(C2=CC=CC(OC)=C2C3=O)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 29 50
DMSO 29 50

Preparing Stock Solutions for Doxorubicin hydrochloride

The following data is based on the product molecular weight 579.99.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.45 mL 17.24 mL 34.48 mL
2.5 mM 0.69 mL 3.45 mL 6.90 mL
5 mM 0.34 mL 1.72 mL 3.45 mL
25 mM 0.07 mL 0.34 mL 0.69 mL

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Product Documents for Doxorubicin hydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Doxorubicin hydrochloride

For research use only

⚠ WARNING: This product can expose you to chemicals including Doxorubicin hydrochloride (Adriamycin), which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov

Citations for Doxorubicin hydrochloride

Customer Reviews for Doxorubicin hydrochloride (1)

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  • 2uM Doxorubicin damaged NRVM cells in 12 hr
    Name: Anonymous
    Assay Type: In Vitro
    Cell Line/Tissue: Heart myocyte
    Verified Customer | Posted 12/12/2017
    Used 2uM Doxorubicin hydrochloride to treat Neonatal rat ventricular myocytes for 12 hr to 24 hr, see clear effect of cell damage.
    Doxorubicin hydrochloride 2252

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