VGLUT1 Antibody (CL2754) - BSA Free

Novus Biologicals | Catalog # NBP2-46627

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2754

Format

BSA Free
View all VGLUT1/SLC17A7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9P2U7, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for VGLUT1 Antibody (CL2754) - BSA Free

Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627] - VGLUT1 Antibody (2754) [NBP2-46627] - Staining in human cerebral cortex and tonsil tissues. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.
Western Blot: VGLUT1 Antibody (CL2754) [NBP2-46627]

Western Blot: VGLUT1 Antibody (CL2754) [NBP2-46627]

Western Blot: VGLUT1 Antibody (2754) [NBP2-46627] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of rat brain shows strong positivity in glutamatergic processes in the molecular and granular layers of cerebellum.
Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry-Paraffin: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry-Paraffin: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry-Paraffin: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
Immunohistochemistry: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of mouse hippocampus shows strong positivity in glutamatergic fibers in the CA2 layer.
Immunohistochemistry: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of mouse brain shows strong positivity in glutamatergic fibers in the molecular and granular layers of the cerebellum.
Immunohistochemistry: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry: VGLUT1 Antibody (CL2754) [NBP2-46627]

Immunohistochemistry: VGLUT1 Antibody (2754) [NBP2-46627] - Staining of rat brain shows strong positivity in glutamatergic fibers in the polymorph layer of dentate gyrus.

Applications for VGLUT1 Antibody (CL2754) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VGLUT1/SLC17A7

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Long Name

Vesicular Glutamate Transporter 1

Alternate Names

BNPI, SLC17A7

Entrez Gene IDs

57030 (Human)

Gene Symbol

SLC17A7

UniProt

Q9P2U7

Additional VGLUT1/SLC17A7 Products

Product Documents for VGLUT1 Antibody (CL2754) - BSA Free

Certificate of Analysis

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Product Specific Notices for VGLUT1 Antibody (CL2754) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

Citations for VGLUT1 Antibody (CL2754) - BSA Free

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Protocols

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FAQs

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