VGLUT1 Antibody (CL2754) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43811

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2754

Format

Azide and BSA Free
View all VGLUT1/SLC17A7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q9P2U7, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for VGLUT1 Antibody (CL2754) - Azide and BSA Free

VGLUT1 Antibody (CL2754) - Azide and BSA Free Western Blot: VGLUT1 Antibody [NBP3-43811]

Western Blot: VGLUT1 Antibody [NBP3-43811]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue
VGLUT1 Antibody (CL2754) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Analysis in human cerebral cortex and tonsil tissues using NBP3-43811 antibody. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.
VGLUT1 Antibody (CL2754) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Staining of human cerebral cortex shows strong positivity in neuropil.
VGLUT1 Antibody (CL2754) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Staining of human tonsil shows no positivity in lymphoid cells as expected.
VGLUT1 Antibody (CL2754) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Staining of mouse brain shows strong positivity in glutamatergic fibers in the molecular and granular layers of the cerebellum.
VGLUT1 Antibody (CL2754) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Staining of rat brain shows strong positivity in glutamatergic fibers in the polymorph layer of dentate gyrus.
VGLUT1 Antibody (CL2754) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Staining of rat brain shows strong positivity in glutamatergic processes in the molecular and granular layers of cerebellum.
VGLUT1 Antibody (CL2754) - Azide and BSA Free Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP3-43811]

Staining of mouse hippocampus shows strong positivity in glutamatergic fibers in the CA2 layer.

Applications for VGLUT1 Antibody (CL2754) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VGLUT1/SLC17A7

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Long Name

Vesicular Glutamate Transporter 1

Alternate Names

BNPI, SLC17A7

Gene Symbol

SLC17A7

Additional VGLUT1/SLC17A7 Products

Product Documents for VGLUT1 Antibody (CL2754) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for VGLUT1 Antibody (CL2754) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

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Protocols

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FAQs

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