VGLUT2 Antibody (N29/29) [FL550] - Chimeric

Novus Biologicals | Catalog # NBP3-52376

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

FL550

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # N29/29
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Product Specifications

Immunogen

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGLUT2 (Uniprot#: Q9JI12) produced recombinantly in E. Coli

Reactivity Notes

Electric Ray

Specificity

Does not cross-react with VGlut1 or VGlut3

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

FL550 Excitation: 550 nm, Emission: 575nm.
Each order is a unique lot. For specialized requirements, please inquire for available options.

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N29/29 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N29/29 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and conjugated.

Applications for VGLUT2 Antibody (N29/29) [FL550] - Chimeric

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS (pH 7.4)

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: VGLUT2

VGLUT2 is a sodium dependent inorganic phosphate cotransporter that is involved in the calcium dependent glutamate release from astrocytes. It is expressed in only a subset of neurons, localized to synaptic vesicles, at synapses exhibiting classical excitatory features. VGLUT2 mRNA is found in brain regions that lack VGLUT1, a related protein also displaying sodium dependent inorganic phosphate cotransporter activity.

Alternate Names

differentiation-associated BNPI, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, differentiation-associated Na-dependent inorganic phosphate cotransporter, DNPI, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, solute carrier family 17 member 6, vesicular glutamate transporter 2, VGLUT2

Gene Symbol

SLC17A6

Additional VGLUT2 Products

Product Documents for VGLUT2 Antibody (N29/29) [FL550] - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for VGLUT2 Antibody (N29/29) [FL550] - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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