VGLUT2 Antibody (N29/29) - Chimeric - BSA Free

Novus Biologicals | Catalog # NBP3-52338

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # N29/29

Format

BSA Free
View all VGLUT2 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGLUT2 (Uniprot#: Q9JI12) produced recombinantly in E. Coli

Reactivity Notes

Electric Ray

Specificity

Does not cross-react with VGlut1 or VGlut3

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N29/29 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N29/29 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.

Scientific Data Images for VGLUT2 Antibody (N29/29) - Chimeric - BSA Free

Immunohistochemistry-Paraffin: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of VGLUT2 with NBP3-52338 at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. NBP3-52338 specifically stains the mossy fiber terminals in the granular layer and climbing climbing fiber terminals in the molecular layer of the cerebellum. Magnification 100X.
Western Blot: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]

Western Blot: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]

Western blotting of rat brain membrane fraction with NBP3-52338 antibody and detected with anti-chicken-HRP. This antibody recognizes endogenous VGLUT2 in the rat brain membrane fraction at ~60 kDa.
Immunohistochemistry-Paraffin: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]

Immunohistochemistry-Paraffin: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of VGLUT2 with NBP3-52338 at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the mossy fiber terminals in the granular layer and climbing climbing fiber terminals in the molecular layer of the cerebellum. Magnification 400X.

Applications for VGLUT2 Antibody (N29/29) - Chimeric - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:400

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS (pH 7.4)

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VGLUT2

VGLUT2 is a sodium dependent inorganic phosphate cotransporter that is involved in the calcium dependent glutamate release from astrocytes. It is expressed in only a subset of neurons, localized to synaptic vesicles, at synapses exhibiting classical excitatory features. VGLUT2 mRNA is found in brain regions that lack VGLUT1, a related protein also displaying sodium dependent inorganic phosphate cotransporter activity.

Alternate Names

differentiation-associated BNPI, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, differentiation-associated Na-dependent inorganic phosphate cotransporter, DNPI, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, solute carrier family 17 member 6, vesicular glutamate transporter 2, VGLUT2

Gene Symbol

SLC17A6

Additional VGLUT2 Products

Product Documents for VGLUT2 Antibody (N29/29) - Chimeric - BSA Free

Certificate of Analysis

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Product Specific Notices for VGLUT2 Antibody (N29/29) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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