Vinculin Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-20859

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (98%), Chicken (93%), Porcine (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Vinculin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.

Localization

Cytoplasm; cytoskeleton; Cell junction; adherens junction; Cell membrane; Peripheral membrane protein; Cytoplasmic side

Marker

Focal Adhesion Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Vinculin Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody [NBP2-20859]

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody [NBP2-20859]

Immunocytochemistry/Immunofluorescence: Vinculin Antibody [NBP2-20859] - Vinculin antibody detects Vinculin protein at focal adhesion by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Vinculin stained by Vinculin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859]

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859]

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859] - Human hepatoma, using Vinculin antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Western Blot: Vinculin Antibody [NBP2-20859]

Western Blot: Vinculin Antibody [NBP2-20859]

Western Blot: Vinculin Antibody [NBP2-20859] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Vinculin Antibody [NBP2-20859]

Western Blot: Vinculin Antibody [NBP2-20859]

Western Blot: Vinculin Antibody [NBP2-20859] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859]

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859]

Immunohistochemistry-Paraffin: Vinculin Antibody [NBP2-20859] - Mouse testis. Vinculin antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Vinculin Antibody - Azide Free

Western Blot: Vinculin Antibody [NBP2-20859] -

Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (NBP2-20859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Vinculin Antibody - Azide Free

Western Blot: Vinculin Antibody [NBP2-20859] -

Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (NBP2-20859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Vinculin Antibody - Azide Free

Western Blot: Vinculin Antibody [NBP2-20859] -

Vinculin antibody detects Vinculin protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody (NBP2-20859) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Vinculin Antibody - Azide Free

Western Blot: Vinculin Antibody [NBP2-20859] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N1N3] (NBP2-20859) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Vinculin Antibody - Azide Free

Western Blot: Vinculin Antibody - Azide Free [NBP2-20859] -

Effects of erastin (2.5 uM) on various oxidative and ferroptosis-related genes in OVCAR-8 (A) and NCI/ADR-RES cells (B) cells following treatment with erastin for 4 h and 24 h. Protein levels for GPX4, NrF2, and NOX4 following treatment with 2.5 uM for 4 and 24 h in OVCAR-8 and NCI/ADR-RES cells (C). * p < 0.05, ** and *** p values < 0.005 and 0.001, respectively, compared to control ( beta -Actin at 4 h and 24 h, respectively). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/16/8666), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Vinculin Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-20859 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Gene Symbol

VCL

UniProt

P18206

Additional Vinculin Products

Product Documents for Vinculin Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Vinculin Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Vinculin Antibody - Azide Free

Customer Reviews for Vinculin Antibody - Azide Free (1)

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Customer Images

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  • Antibody works good
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 12/10/2024
    WB_Liver lysate_Vinculin
    The antibody was diluted 1:3000 in 1X TBST. Multiple bands were observed.
    Vinculin Antibody - Azide Free NBP2-20859

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Protocols

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FAQs for Vinculin Antibody - Azide Free

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  • Q: Do you have any 100 coda or bigger controls?

    A:

    I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

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