Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Vinculin Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 23 amino acids near the center of human Vinculin. Amino Acid Sequence: VREAFQPQEPDFPPPPPDLEQLR

Specificity

Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist. Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD). Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD). NBP2-41274 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Vinculin Antibody - BSA Free

Western Blot: Vinculin AntibodyBSA Free [NBP2-41274]

Western Blot: Vinculin AntibodyBSA Free [NBP2-41274]

Western Blot: Vinculin Antibody [NBP2-41274] - Western Blot Validation in PC3 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: Vinculin NBP2-41274 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Vinculin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody - BSA Free [NBP2-41274] -

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody - BSA Free [NBP2-41274] - Validation of Vinculin in Jurkat Cells. Immunocytochemical analysis of Jurkat cells using anti-Vinculin antibody at 5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: Vinculin AntibodyBSA Free [NBP2-41274]

Western Blot: Vinculin AntibodyBSA Free [NBP2-41274]

Western Blot: Vinculin Antibody [NBP2-41274] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41237 (2 ug/mL), Vinculin from Company B (1 ug/mL), Vinculin NBP2-41274 (1 ug/mL), and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Vinculin AntibodyBSA Free [NBP2-41274]

Western Blot: Vinculin AntibodyBSA Free [NBP2-41274]

Western Blot: Vinculin Antibody [NBP2-41274] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41274 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Vinculin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody - BSA Free [NBP2-41274] -

Immunocytochemistry/ Immunofluorescence: Vinculin Antibody - BSA Free [NBP2-41274] - Validation of Vinculin in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Applications for Vinculin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry

5 ug/mL

Immunocytochemistry/ Immunofluorescence

5-20 ug/mL

Western Blot

0.5-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Gene Symbol

VCL

Additional Vinculin Products

Product Documents for Vinculin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Vinculin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Vinculin Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Vinculin Antibody - BSA Free

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  • Q: Do you have any 100 coda or bigger controls?

    A: I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

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