Vitronectin Antibody (ST49-02)

Novus Biologicals | Catalog # NBP2-67511

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ST49-02 expressed in HEK293
View all Vitronectin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Vitronectin aa 429-478 / 478. (SwissProt: P04004 Human; SwissProt: P29788 Mouse; SwissProt: Q3KR94 Rat)

Localization

Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Vitronectin Antibody (ST49-02)

Western Blot: Vitronectin Antibody (ST49-02) [NBP2-67511]

Western Blot: Vitronectin Antibody (ST49-02) [NBP2-67511]

Western Blot: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of Vitronectin on different lysates using anti-Vitronectin antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver Lane 2: Human serum
Immunocytochemistry/ Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunocytochemistry/ Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Vitronectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-39, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunocytochemistry/ Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunocytochemistry/ Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunocytochemistry/ Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded human liver cancer tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded human liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded mouse liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded mouse skin tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]

Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Vitronectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-39, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Vitronectin Antibody (ST49-02)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:400

Western Blot

1:1000-1:5000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vitronectin

Vitronectin also called serum spreading factor or complement S-protein, is a member of the pexin family (1). Found in serum and in tissues, Vitronectin exist in two forms; as a single chain (75kDa) and as a clipped form composed of two chains (65kDa and 10kDa). Vitronectin promotes cell adhesion, spreading and migration through specific interactions with cellular integrins, and inhibits cytolysis by the complement C5b-9 complex (2-4). Vitronectin also interacts with several critical proteins that regulate thrombosis and fibrinolysis. Vitronectin is phosphorylated by PKA and CKII (5).

Alternate Names

Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN

Gene Symbol

VTN

Additional Vitronectin Products

Product Documents for Vitronectin Antibody (ST49-02)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Vitronectin Antibody (ST49-02)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Citations for Vitronectin Antibody (ST49-02)

Customer Reviews for Vitronectin Antibody (ST49-02)

There are currently no reviews for this product. Be the first to review Vitronectin Antibody (ST49-02) and earn rewards!

Have you used Vitronectin Antibody (ST49-02)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies