VMAT2 Antibody (OTI9E11)

Novus Biologicals | Catalog # NBP1-47977

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9E11
View all VMAT2 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SLC18A2 expression vector.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VMAT2 Antibody (OTI9E11)

Western Blot: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Western Blot: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - VMAT2 protein level by using anti-VMAT2 (9E11) antibody in the striatum of autopsied human brain.
Immunocytochemistry/ Immunofluorescence: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Immunocytochemistry/ Immunofluorescence: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Immunocytochemistry/Immunofluorescence: VMAT2 Antibody (OTI9E11) [NBP1-47977] - VMAT2 Antibody (9E11) [NBP1-47977] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VMAT2.
Immunohistochemistry-Paraffin: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Immunohistochemistry-Paraffin: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Immunohistochemistry-Paraffin: VMAT2 Antibody (9E11) [NBP1-47977] - Staining of paraffin-embedded Human pancreas tissue using anti-VMAT2 mouse monoclonal antibody.
Flow Cytometry: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Flow Cytometry: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Flow Cytometry: VMAT2 Antibody (9E11) [NBP1-47977] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VMAT2 antibody, and then analyzed by flow cytometry.
Western Blot: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Western Blot: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VMAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VMAT2.
Western Blot: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Western Blot: VMAT2 Antibody (OTI9E11) [NBP1-47977]

Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti SLC18A2 monoclonal antibody.(1:200).

Applications for VMAT2 Antibody (OTI9E11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 5 using NBP1-47977 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VMAT2

The vesicular monoamine transporter acts to accumulate cytosolic monoamines into synaptic vesicles, using the proton gradient maintained across the synaptic vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs,including reserpine and tetrabenazine.

Long Name

Vesicular Monoamine Transporter 2/Solute Carrier Family 18, Member 2

Alternate Names

MNAT, SLC18A2, SVAT, SVMT, VAT2

Entrez Gene IDs

6571 (Human)

Gene Symbol

SLC18A2

UniProt

Q05940

Additional VMAT2 Products

Product Documents for VMAT2 Antibody (OTI9E11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VMAT2 Antibody (OTI9E11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Markers

Citations for VMAT2 Antibody (OTI9E11)

Customer Reviews for VMAT2 Antibody (OTI9E11) (1)

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  • VMAT2 Antibody (OTI9E11)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 08/19/2021
    VMAT2 Antibody in mouse brain.
    VMAT2 Antibody (OTI9E11) NBP1-47977

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Protocols

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