Von Willebrand Factor Antibody - BSA Free

Novus Biologicals | Catalog # NB600-586

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Canine, Feline

Cited:

Human, Mouse, Canine, Feline

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Von Willebrand Factor Primary Antibodies »

Product Specifications

Immunogen

Purified antisera of the Von Willebrand Factor (factor VIII R:ag) related antigen in plasma.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31738896). Feline reactivity reported in scientific literature (PMID:31616579).

Localization

Secreted. Localized to storage granules.

Marker

Endothelial Cell Marker

Specificity

The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin's or ethanol-fixed and paraffin-embedded tissue sections.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Von Willebrand Factor Antibody - BSA Free

Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, catalog # 5860461; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus catalog # NB600-586) at 1:500 dilution for 1 hour at room temperature followed by incubation with the anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).
Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.
Immunohistochemistry-Frozen: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry-Frozen: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry-Frozen: Von Willebrand Factor Antibody [NB600-586] - Canine brain/spinal cord. VWF antibody in green. A pericyte marker in red. Alexa Fluor conjugated secondary antibodies were used for visualization. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586]

Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.

Applications for Von Willebrand Factor Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000
Application Notes
IHC-P: We recommend proteolycic antigen retrieval. After deparaffinization treat with 0.1% Pronase in PBS for 5-10 minutes at 37 degrees C. Alternatively, digest with 0.1% Trypsin in PBS for 25 minutes at 37 degrees C. Gently rinse in PBS. This Von Willebrand Factor Antibody is validated for IHC-Fr from a verified customer review.

Reviewed Applications

Read 3 reviews rated 4.3 using NB600-586 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

10 mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Von Willebrand Factor

Human Von Willebrand Factor(factor VIII R:Ag) is a 270 kDa multimeric plasma gylcoprotein. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes.

Alternate Names

coagulation factor VIII VWF, F8, F8VWF, von Willebrand factor, VWD, vWF

Entrez Gene IDs

7450 (Human)

Gene Symbol

VWF

UniProt

P04275

Additional Von Willebrand Factor Products

Product Documents for Von Willebrand Factor Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Von Willebrand Factor Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Von Willebrand Factor Antibody - BSA Free

Customer Reviews for Von Willebrand Factor Antibody - BSA Free (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Von Willebrand Factor Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: brain and spinal cord
    Species: Canine
    Verified Customer | Posted 12/16/2019
    This multi-immunolabeled image was acquired by fluorescence microscopy. vWF antibody (NB600-586) (green), a pericyte marker (red).
    Alexa Fluor conjugated secondary antibodies were used.
    Von Willebrand Factor Antibody - BSA Free NB600-586
  • Von Willebrand Factor Antibody
    Name: Yukio Sasaki
    Application: Immunofluorescence
    Sample Tested: Endothelial
    Species: Mouse
    Verified Customer | Posted 01/05/2018
    vWF staning is detected in a mouse vascular endothelial cell line, UV♀2 (dilution: 1:50 [green], DAPI: blue).
    Von Willebrand Factor Antibody - BSA Free NB600-586
  • Von Willebrand Factor Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Connective tissue
    Species: Feline
    Verified Customer | Posted 08/01/2017
    Immunofluorescence on 4%PFA fixed section. Green-vWF(NB600-586), Blue-DAPI. Vessels are immunolabeled.
    Von Willebrand Factor Antibody - BSA Free NB600-586

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Protocols

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FAQs for Von Willebrand Factor Antibody - BSA Free

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  • Q: I have Von Willebrand Factor Antibody (NB600-586) being stored at -20 degrees Celsius and was wondering what the proper way to thaw them prior to use?

    A: There is no special method. You can just take it out of the freezer and let it thaw on the lab bench. We suggest you could check it in an hour; it's not a large volume so shouldn't take very long.

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