VPS11 Antibody

Novus Biologicals | Catalog # NB100-2412

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all VPS11 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HALQEKDTQTKLE corresponding to internal region according to NP_068375.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for VPS11 Antibody

Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412]

Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412]

Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412] - (4ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412]

Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412]

Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412] - 3.8ug/ml staining of paraffin embedded Human Kidney-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for VPS11 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave an approx. 75 kDa band in human duodenum, colon and placenta lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108 kDa band according to NP_068375.3. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows brushborder staning in PCT cells and human pancreas shows cytoplasm staining of beta cells in islets of langerhans.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS11

VPS11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Alternate Names

hVPS11, PEP5, RING finger protein 108, RNF108END1, vacuolar protein sorting 11 (yeast homolog), vacuolar protein sorting 11 homolog (S. cerevisiae), vacuolar protein sorting protein 11, vacuolar protein sorting-associated protein 11 homolog

Entrez Gene IDs

55823 (Human); 71732 (Mouse); 315600 (Rat)

Gene Symbol

VPS11

UniProt

NP_068375.3 (Human)

Additional VPS11 Products

Product Documents for VPS11 Antibody

Certificate of Analysis

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Product Specific Notices for VPS11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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